High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The 1290 Infinity Flexible Cube is an auxiliary module that allows flexible online SPE applications by supporting up to two Agilent Quick Change Valve drives for online SPE valves and a built-in pump to facilitate enhanced flushing capabilities and to reduce carryover.
LC Manual Syringes with PTFE-Tipped Plungers
ZirChrom Separations Inc. (Anoka, MN) provides a full line of zirconia-based HPLC columns for chromatographic method development. ZirChrom HPLC columns offer unique selectivity, exacting reproducibility, high efficiency and ultra-stability. ZirChrom columns have proven utility over a wide range of pharmaceutical compounds. The columns may be used at both ambient and elevated temperatures (which helps enhance LC/MS sensitivi…
A Sensitive solution for HPLC, fast-LC and CE systems High Sensitivity: 10 times to 100 times greater than conventional fluorescence and up to 100,000 times more sensitive than UV detection. Versatility: 14 interchangelable different lasers availables for any application Modularity : Adaptable to CE and HPLC systems including Fast-LC, Capillary LC and nanoLC.Selectivity: Low noise and interference molecules will be detected.…
The ZORBAX Eclipse AAA high efficiency HPLC column rapidly separates amino acids following an updated and improved protocol. Total analysis from injection to injection can be achieved in as little as 14 min (10 min analysis time) on the short, 7.5 cm column and 24 min (16 min analysis time) on the 15 cm column length.Exceptional sensitivity (5 - 50 pmol with DAD, FLD) and reliability are achieved using both OPA and FMOC deriv…
Exceptional peak symmetry and resolution Based on next-generation ultra pure silica Outstanding pH stability Ideal first choice for new method developmentProduct detail:Hypersil GOLD columns are a new LC and LC/MS column offering unsurpassed peak shape. Based on highly pure silica, Hypersil GOLD columns provide the ultimate in symmetrical peaks, even when analyzing compounds that give notoriously poor peak shape on traditional…
Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phas…
The 1260 Infinity II High-Temperature GPC System can provide information about all of today’s engineering polymers such as polyolefins, which are one of the most popular polymers with numerous applications. This level of detail helps with development and to predict how a given batch will process and perform in the final product.
By combining the signals from two diode array detectors with different path length Max-Light flow cells, the 1290 Infinity II High Dynamic Range DAD Solution allows you to detect and quantify all components in your sample in a single run. The 30 times wider linear UV range offered by the high dynamic range detection expands your UV detection capacity. The system is ideal for analysis of mixtures with widely different concentr…
CoulArray® - The Only Coulometric Array Detector for HPLC ESA's pioneering electrochemical technology has made possible the multi-sensor detector for HPLC. Measure multiple analytes simultaneously, including those that are chromatographically unresolved. Only ESA's unique coulometric array detection provides: Sensitivity to femtomole levels 3D resolution of co-eluting peaks Qualitative characterization of peaks Simpl…
ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because…
The Finnigan Surveyor Plus family of HPLC instruments is specifically optimized for the most demanding HPLC and LC/MS applications. Short cycle times, high sample capacity, low flow rate performance, and patented LightPipeTM technology have been integrated into one complete system for high-throughput sample processing environments.
The EXP® Guard Column cartridge system is rated to 15,000 psi with hand-tight replaceable cartridges. This unique design connects directly to any UHPLC column with the auto-adjusting Titanium Hybrid Ferrule and EXP® Fittings, providing repeated zero-dead-volume connections and easy adjustability between various column types. Free-Turn¨ architecture allows the user to change cartridges by hand without breaking fluid connections…
The Agilent open-systems approach to laboratory informatics allows our customers to select the best hardware and software to meet their needs. The instrument control program consists of two components to enable multivendor control in chromatography data systems (CDS). Instrument control framework (ICF) and RapidControl.Net (RC.Net) standardize how chromatography data systems (CDS) interact with analytical instruments.…
The Shim-pack XR-ODS, which was developed with the aim of increasing the speed attained under standard column pressure conditions, has been joined by the Shim-pack XR-ODS II, which was developed in order to facilitate high-speed, high-separation analysis for a wider range of applications, such as those involving methanol mobile phases, by using a new long column. The Shim-pack XR-ODS II is a high-separation analysis column tha…
Adding a syringe filtration step prior to injection not only helps to ensure more consistent and reliable results; it also helps protect delicate instruments and prolongs column life. Available in sizes and membrane types to meet any application need, Thermo Scientific™ Titan3™ Syringe Filters provide cleaner sample extracts by removing interfering materials and fine particles down to the rated pore size. Titan3 Nylon Syringe…
The 1290 Infinity II LC System is an ultra-high performance LC that has market-leading sample capacity and is designed for the lowest dispersion for the most challenging ultra-HPLC experiments. The 1290 Infinity II LC system is designed to be both a HPLC and high-end UHPLC system in a single flow path, giving you robust and excellent performance no matter what application you are running while seamlessly integrating into your…
Agilent offers high- and ultrahigh-performance LC systems customized for applications from routine analysis to specialized workflow solutions. Our preparative HPLC systems provide superior sample purification with excellent recovery.
The LC-4000 Series is the latest in a long history of innovative HPLC systems developed by JASCO reaching all the way back to the start of commercial HPLC in the early 1970s. The concept of the integrated LC-4000 series HPLC provides key separation platforms at 50MPa, 70MPa and 130MPa which correspond to conventional HPLC, the increasingly popular Rapid Analysis (RHPLC) and sub 2um UHPLC, respectively. Each platform is suppl…
Using the advanced Czerny- Turnermonochromator design with optimization of the optical sys tem, baseline change due to refrac tive index is minimized.Baseline fluctuation from changes in environmental temperature is reduced with a temperature - controlled cell, baseline drift caused by temperature fluctuation in the light source is also reduced with a temperature controlled light source. The result is excellent stability with…
The Refractive Index detectors include the optical system mounted in a precisely temperature-regulated housing to eliminate the influence of changes in ambient temperature.The RI-4030 and 4035 detectors are fully stable just one hour after powering on. The RI-4030 can be used for both analytical and preparative scales. In preparative mode the maximum flow rate is 120 ml/min. The RI-4035 is a dedicated semi-micro RI detector wi…
Thermo Scientific Accela - HPLC and U-HPLC in a Single System The Thermo Accela™ high-speed chromatographic system provides fast, efficient chromatographic separations over an expansive range of flow rates and pressures. Thermo Accela is designed to optimize performance of sub-two micron particle columns, providing seamless operation spanning conventional LC pressures from short LC columns, up to 15,000 psi for long-column sep…
High performance Gel Filtration column from Shodex® Chemistry: Diol (OH) Solvent: H2O Product Exclusion limit (Proteins): 700,000 Product Exclusion limit (Pullulan): 170,000 Product Particle size, µm: 5 Product pH range: 3 - 7.5 Product Plate number (TP/Column): >21,000 Product Pore Size Maximum: 1,000 Product Size (I.D. x Length, mm): 8.0 x 300
As an alternative to conventional columns, PharmaFluidics offers micromachined nano-LC columns or micro Pillar Array Columns (µPAC™). These µPAC™ columns display exceptional separation properties with unprecedented peak capacity and narrow peak shapes at low operating back-pressure. µPAC™ column, 200 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.
The HTEC-510 is user friendly, provides worry free maintenance, can be easily switched between methods, and most importantly has superb sensitivity. Ultra-High Sensitivity Electrochemical Detection The best advantage of using electrochemical (EC) detection is the sensitivity.The combination of the HTEC-510, HPLC-Electrochemical Detector and the appropriate applications will easily ensure 160 amol sensitivity for dopamine…
