Epigenomic Library Preparation Products & Reviews

The MethylEdge™ System provides an efficient method to rapidly perform bisulfite conversion with minimal DNA fragmentation, giving you more intact, converted DNA for downstream applications.   Start with as little as 100pg of input DNA for those precious, limited samples. Use the MethylEdge™ System with other DNA Methylation Workflow reagents to perform sensitive DNA methylation analysis on many sample types. The rapi…

Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powe…

The EZ-Zyme™ Chromatin Prep kit allows ChIP analysis at nucleosome resolution by performing complete or partial digestions with a proprietary enzymatic cocktail to obtain chromatin fragments of on average one to a few nucleosomes in length. Compared to sonication, this procedure not only allows mapping the protein-DNA association at a higher resolution, but also renders the subsequent immunoprecipitation more efficient due to…

The CpGenome™ DNA Modification Kit is used to create methylation-specific sequence alterations in any DNA sample. Using chemical treatment, the kit converts all unmethylated cytosine residues to uracil. Methylated cytosine residues are unaltered in this process. Therefore, the chemically-treated DNA sequence will differ only if it was originally unmethylated in vivo. After sequence conversion, methylation specific PCR is emplo…

The CpGenome™ Fast DNA Modification Kit contains the reagents for the initial bisulfite modification of the DNA required for both methodologies.

ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP…

The iDeal ChIP Kit for Transcription Factors.Diagenode’s iDeal ChIP-seq Kit for Transcriptions Factors is a highly specialized solution for robust ChIP-seq results transcription factors. Unlike competing solutions, our kit utilizes a highly optimized protocol and is backed by validation with a broad number and range of transcription factors. The kit provides high yields with excellent specificity and sensitivity. Advantages ov…

The Magna ChIP HiSens kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating…

The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methylbinding domain) protein . This procedure has first been optimized on the SX-8G IP-Star Automated System ([page product SX-8G IP-Star]. Afterwards, this method has been adapted to a manual protocol, which has been inspired by the same reproducibility standards provided by the automated MethylCap assay. Allows for fractionati…

The PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results. Features & Benefits • Complete set of materials for up to…