Reverse Transcriptase Products & Reviews

UltraScript® 2.0 cDNA Synthesis Kit is a 2-tube kit containing all the components required for fast, reliable and unbiased cDNA synthesis of the most challenging RNA sample types.

UltraScript® 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase designed for cDNA synthesis of the most challenging RNA sample types. This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55 °C, giving improved specificity, higher cDNA yields and more full length cDNA product.

UltraScript® cDNA Synthesis Kit is an easy-to-use 2-tube system specifically developed to generate cDNA for use in real-time PCR. The kit provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

PCRBIO 1-Step Go RT-PCR Kit is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube. The advanced buffer system, reverse transcriptase and hot start polymerase give highly specific and ultra-sensitive 1-step RT-PCR from any RNA template including mRNA, total RNA and viral RNA sequences.

IsoFast® Bst 1-Step Mix is a dual enzyme system for rapid and sensitive isothermal amplification of RNA targets in one step.

The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR. The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC). Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.

Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

The Agilent AffinityScript Multiple Temperature Reverse Transcriptase (RT) system is optimized to produce high cDNA yields over the broadest temperature range. During analysis the multi-temp RT device also ensures high affinity to primer-template complexes. cDNA can be synthesized in amounts up to 20 kb.

Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The Just cDNA Double-Stranded cDNA Synthesis Kit generates high-quality, blunt-ended cDNA, perfect for use in non-directional cDNA libraries.  Makes blunt double-stranded cDNA  Can be used for in vitro transcription  Perfect for nondirectional cDNA libraries

Hiscript III RT SuperMix for PCR (+gDNA wiper) is an upgraded vesion of Hiscript III Q RT Supermix for GPCR(+gDNA wiper), induding Hiscript III Reverse Transcriptase, a new generation of reverse transcriptase with optimized Buffer. 

HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. Compared with the last generation reverse transcriptase, the thermo-stability of this product is significantly improved. 

MedixMDx Fast Bst RT Mix contains a Bst DNA polymerase that displays high strand displacement activity, and an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. MedixMDx Fast Bst RT Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature.  

Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments) for the following applications: Synthesis of first-strand cDNA for use in subsequent amplification reactions RT-PCR of GC-rich RNA templates Cy3, Cy5, DIG, biotin, and a…

The Thermo Scientific Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increas…

Thermo Scientific Verso 1-step RT-qPCR Kits for probe chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment…

Thermo Scientific Verso 1-step RT-qPCR Kits for SYBR Green chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I trea…

Thermo Scientific Verso 1-Step RT-PCR system is a complete set of RT-PCR reagents that combines everything needed for RT-PCR. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. Verso also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to c…

Get more cDNA in less time with CycleScript Reverse Transcriptase. CycleScript is a versatile reverse transcriptase - applicable to both conventional Reverse Transcription and Cyclic Reverse Transcription (Cyclic RT, patent pending – for cDNA amplification). It features high activity across a wide range of temperatures from 37 to 55°C; therefore, reverse transcription is carried out like PCR. The Cyclic RT reaction is composed…

Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme is able to use an RNA molecule as a template and synthesize a double-strand DNA. M-MLV Reverse Transcriptase is isolated from a E.coli strain containing a recombinant clone. It is ideal for use of first-strand synthesis cDNA from RNA molecules and for cDNA synthesis for Reverse Transcriptase PCR and qRT-PCR.