Reverse Transcriptase
25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 200 µl No-RT control supermix; 12.5 µl DNase; 150 µl DNase buffer
100 x 20 µl reactions, 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 400 µl No-RT control supermix; 50 µl DNase; 150 µl DNase buffer
50 x 50 µl reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water
25 x 20 µl reactions, 100 µl 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix
100 x 20 µl reactions, 4 x 100 µl 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix
25 x 20 µl reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
100 x 20 µl reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
250 x 50 µl reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water
EpiScript™ Rnase H- Reverse Transcriptase (EpiScript RT) is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.
25 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 25 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water
100 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 100 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water
500 x 20 µl reactions; bundle of 5 boxes of 100 reaction kit; includes 2,000 µl Reliance Select 5X reaction mix, 500 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water
Hiscript III RT SuperMix for PCR (+gDNA wiper) is an upgraded vesion of Hiscript III Q RT Supermix for GPCR(+gDNA wiper), induding Hiscript III Reverse Transcriptase, a new generation of reverse transcriptase with optimized Buffer.
HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. Compared with the last generation reverse transcriptase, the thermo-stability of this product is significantly improved.
MedixMDx Fast Bst RT Mix contains a Bst DNA polymerase that displays high strand displacement activity, and an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. MedixMDx Fast Bst RT Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature.
Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments) for the following applications: Synthesis of first-strand cDNA for use in subsequent amplification reactions RT-PCR of GC-rich RNA templates Cy3, Cy5, DIG, biotin, and a…
The Thermo Scientific Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increas…
Thermo Scientific Verso 1-step RT-qPCR Kits for probe chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment…
Thermo Scientific Verso 1-step RT-qPCR Kits for SYBR Green chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I trea…
Thermo Scientific Verso 1-Step RT-PCR system is a complete set of RT-PCR reagents that combines everything needed for RT-PCR. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. Verso also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to c…
Get more cDNA in less time with CycleScript Reverse Transcriptase. CycleScript is a versatile reverse transcriptase - applicable to both conventional Reverse Transcription and Cyclic Reverse Transcription (Cyclic RT, patent pending – for cDNA amplification). It features high activity across a wide range of temperatures from 37 to 55°C; therefore, reverse transcription is carried out like PCR. The Cyclic RT reaction is composed…
Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme is able to use an RNA molecule as a template and synthesize a double-strand DNA. M-MLV Reverse Transcriptase is isolated from a E.coli strain containing a recombinant clone. It is ideal for use of first-strand synthesis cDNA from RNA molecules and for cDNA synthesis for Reverse Transcriptase PCR and qRT-PCR.
