Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The GoTaq®Real-Time PCR Systems are ready-to-use 2X master mixes optimized for quantitative PCR in dye (using BRYT(R) Green Dye) or hydrolysis probe detection format. The 1-Step and 2-Step RT-qPCR Master Mixes contain GoScript™ Reverse Transcriptase for efficient first-strand cDNA synthesis. GoTaq Real-Time PCR Systems Features & Benefits: Higher fluorescence, often resulting in earlier Cqs Direct su…
These automated pipetting systems are perfectly suited for PCR and qPCR setup, nucleic acid purification as well as low-throughput NGS library preparation yet they retain the flexibility for use as open platforms for diverse automated liquid handling applications. The epMotion 5073 series automates and simplifies traditionally complex, labor-intensive pipetting tasks, saving time and improving the reliability and reproducibili…
The PowerPlex® Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use. The PowerPlex® Fusion System provides all of the materials needed for co-amplification and five-color fluorescent detection of 24 loci (23 STR loci and Amelogenin), including the CODIS core loci and the European Standard Set (ESS) loci. With 24 loci, the sys…
The Applied Biosystems 7300 Real-Time PCR System gives you advanced, multicolor real-time PCR capabilities with exceptional budget sensitivity. Run time of less than 2 hours Four-color detection enables you to perform gene expression analysis, pathogen quantitation, SNP genotyping, and plus/minus assays with internal positive controls Study gene expression, detect and quantitate pathogens, perform allelic discrimination…
The DNA LoBind Tube finally provides the genomics user with the certainty that sample loss of genetic material caused by interaction with the plastic surface of the tube will be insignificant. With the combination of the purest selected types of polypropylene and specially developed production technologies, a tube has been developed that ensures nearly 100% recovery of DNA/RNA. A comparison of the recovery rates of various p…
Robotic workstation for fully automated nucleic acid preparation and filling of LightCycler® Capillaries, 96-well PCR plates, and tubes suitable for the most commonly used PCR instruments Benefits Save time with rapid, fully automated isolation of high-quality DNA, RNA, and mRNA. Obtain reliable, reproducible results. Benefit from high flexibility in sample type and volume, as well as elution volume and post-elution p…
TaqMan® Gene Expression Assays are a comprehensive collection of over 1,000,000 probe and primer sets for quantitative gene expression analysis of 13 species including human, mouse, rat, Arabidopsis, Drosophila, and C. elegans genes. These assays let you quickly, easily, and affordably perform precise gene expression studies using real-time PCR. TaqMan®-based assays provide unmatched sensitivity, specificity, and reproducibi…
With over 4.5 million SNP assays available, including 3.5 million HapMap SNPs, 70K cSNPs, and 160,000 validated assays, Applied Biosystems SNP Genotyping Assays make it easy to perform SNP genotyping studies with the precision of TaqMan® reagent-based chemistry. These assays provide SNP detection capabilities with high-confidence results. The large selection of SNP assays helps advance your screening, association, candidate…
Applied Biosystems GeneAmp® Fast PCR Master Mix's sensitive, pre-mixed hot-start chemistries reduce PCR reaction time to as little as 25 minutes when used with the 9800 Fast PCR System. • Pre-mixed hot-start chemistry enables you to set up your experiments quickly. • Detect a single copy of a gene in as little as 10 ng of DNA. • Get reproducible, specific, and sensitive results.
Extensor Long Range Enzyme Blend is an optimized mix of ThermoPrime* DNA Polymerase and a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate long PCR products, up to 20kb. This makes the enzyme blend ideal for robust full-length amplification of long range sequences. ■ Amplifies targets up to 20kb in length ■ Unique enzyme blend and optimized buffer combination delivers increased accu…
The PX1 PCR plate sealer includes the heat sealing instrument with power cord, a single plate support block that fits both 96- and 384-well plates, and a sealing frame to aid in heat sealing.
The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimi…
Introducing the LightCycler® 96 Real-Time PCR System What if you had the power to get your qPCR data fast, reliably and with less effort? Well, now you can. The result of more than a decade of Roche experience developing high-performance qPCR systems is now accessible for any research lab. The LightCycler® 96 System delivers data that makes you a publication superhero: • Fast precision thermal cycling • Leading-edge optics fo…
The TaqMan® One-Step RT-PCR Master Mix Reagents Kit, which combines the reverse transcription and amplification steps, permits excellent results even when quantifying minute amounts of RNA. Set-up is quick, easy, and minimizes risk of sample crossover and contamination, making this kit well-suited for all high-throughput applications. • Optimized for high sensitivity and yield • Combined RT (reverse transcription) and amplific…
Syzygy FlashTaq 2x Master Mix is a ready-to-use 2x mixture of FlashTaq, dNTPs, buffers and magnesium for efficient PCR amplification. Just add template DNA, primers, and water. FlashTaq Hot-Start DNA Polymerase is a chemically modified Taq polymerase designed for reducing nonspecific DNA amplification including primer dimers. The activiation time for FlashTaq is only 2 minutes at 95°C, which is faster than that for leading a…
rEVAlution 2X qPCR Master Mix is a ready to use hot-start qPCR master mix with EvaGreen® and FlashTaq, a hot start Taq polymerase. A 25μM ROX reference dye is supplied separately for use with some real time instruments. The master mix is optimized to work with both fast cycling conditions and regular three step cycling conditions. Available in 3 pack sizes: 100 20µL Reactions, 500 20µl reactions and 1,000 20µl reactions.
PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR. Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension,…
Rapid and accurate detection of foodborne pathogens
Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…
1 ml, one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 100 x 20 µl reactions
SensiFAST™ cDNA Synthesis Kit has been developed to generate high-quality first strand cDNA from total RNA template. SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first strand cDNA synthesis for use in real-time PCR studies (fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, reg…
SensiFAST™ Probe Hi-ROX Kit and SYBR® Hi-ROX Kit have been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Kits delivers fast, highly-specific and ultra-sensitive real-time PCR.SensiFAST™ Probe Hi-ROX Kit has been d…
