PCR and Thermal Cycling Products & Reviews

Optimized PCR master mix: Just add template, primers and water

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so tha…

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Mast…

Twist Bioscience, the leader in synthetic DNA with unparalleled precision at scale, is redefining targeted sequencing performance with the Twist Human Core Exome Kit. This kit includes library preparation and enrichment components, allowing the flexibility to lower sequencing costs, increase sample throughput, and achieve a higher depth of coverage across target regions with uncompromising quality.

Positive controls provide quality control measures for the verification and validation for point-of-care diagnostic tests including both next-generation sequencing (NGS) and reverse transcriptase-polymerase chain reaction (RT-PCR) assays. Twist offers fully synthetic SARS-CoV-2 RNA controls, available for dozens of distinct reference sequences.

96-well Real-Time PCR instrument with 10.2” touchscreen interface, 6 dye channel filters

96-well real-time PCR instrument for in-vitro diagnostic (IVD) use

qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific and ultra-sensitive probe-based real-time RT-PCR.

UltraScript® Reverse Transcriptase is a robust and thermostable modified MMLV reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.

PCRBIO Taq DNA Polymerase is an affordable, versatile and robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. An enhanced 12-step purification strategy together with an optimised buffer system enable PCRBIO Taq DNA Polymerase to amplify with the highest speed, yield and specificity.

qPCRBIO SyGreen® Mix combines a proprietary non-inhibiting intercalating dye with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive, and reproducible real-time PCR.

PCRBIO Taq Mix Red is an affordable, versatile and robust 2x mix for all your everyday PCR applications including genotyping, screening and library construction. The mix contains a red dye allowing reactions to be directly loaded onto agarose gels.

PCRBIO Classic Taq is a highly purified recombinant Taq DNA Polymerase for all your everyday PCR applications including genotyping, screening and library construction. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA.

VeriFi® Mix Red is a versatile and robust high fidelity 2x ready-mix engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. The mix includes a convenient red dye for direct gel loading.

VeriFi® Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. 

Our universal probe kit is designed to give superior sensitivity and specificity in all probe-based real-time PCR assays, including TaqMan, Scorpions and molecular beacon probes. Whether your application is for a singleplex or multiplex expression study or for genotyping and allelic discrimination analyses, qPCRBIO Probe Blue Mix is the robust choice for all your probe-based real-time PCR needs.

Clara® Probe Mix is the newest solution for qPCRs. With clear and consistent results, this cutting-edge qPCR mix will streamline your real-time PCR workload no matter what the application.

Highly transparent adhesive sealing tape with pressure- and heat-activated adhesive

Thin walled PCR-Tube for optimal heat transfer during PCR

Manufactured from medical grade polypropylene

Primer and probe premixed assays for analyzing gene expression in any species using fluorescently labeled 5' nuclease probes

Obtain high quality results with this thermostable probe-based qPCR master mix

IDT offers a wide array of primer and probe sets, as well as plasmid controls, for the identification of SARS-CoV-2 (2019-nCoV). Primers and probes are manufactured in a template-free environment and certified template-free to cycle 45 by NTC testing.