Protein Labeling Kits Products & Reviews

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Protein Labeling Kits

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EZ-Link™ NHS-LC-LC-Biotin

Thermo Fisher Scientific

Thermo Scientific EZ-Link NHS-LC-LC-Biotin is a doubly long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for intracellular labeling.

4.0/5.0
|1 Review

G-LISA™ Small G-protein Activation Assays

Cytoskeleton Inc.

The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemilu…

4.3/5.0
|1 Review

Quick Western Kit

LICORbio

The Quick Western Kit provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step.

0.0/5.0
|0 Reviews
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IRDye 680RD Infrared Dyes

LICORbio

IRDye 680RD is the near-infrared fluorescent dye of choice for applications in the 700 nm wavelength, especially Western blots, cell-based assays such as the In-Cell Western assay, and small animal imaging experiments.

0.0/5.0
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IRDye Click Chemistry Reagents

LICORbio

IRDye® Click Chemistry reagents (DBCO, Alkyne, and Azide) offer convenient, versatile and reliable methods for biomolecule labeling. With a variety of in vitro and in vivo applications, from biomarker isolation to assay development.

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Tyrosine Kinase Assay Kit, Colorimetric Detection

Merck

This non-radioactive kinase assay kit is a two-stage peptide phosphorylation/colorimetric detection assay kit. In the first stage, a biotinylated substrate peptide containing tandem repeats of Poly (Glu4-Tyr) is incubated with a tyrosine kinase enzyme sample in the presence of non-radioactive ATP and a Mn2+/Mg2+ co-factor cocktail. The kinase assay can be performed with substrate peptide either in solution, or bound to the wel…

0.0/5.0
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Histone Deacetylase Assay Kit (HDAC)

Merck

This kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAC inhibitor sodium butyrate is provided to demonstrate specificity of the deacetylase activity.

0.0/5.0
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HDAC Assay Kit, fluorometric detection

Merck

This assay is a simple two-step procedure performed in a microtiter plate. In the first step samples are incubated with the HDAC assay substrate, allowing deacetylation of the fluorometric substrate. Next, Activator Solution releases a fluorophore from the deacetylated substrate or standard.

0.0/5.0
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Caspase 3 Colorimetric Activity Assay Kit, DEVD

Merck

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a s…

0.0/5.0
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GFP-/RFP Booster

Chromotek GmbH

GFP/RFP-Booster – enhance your fluorescent fusion protein ChromoTek’s Booster are very small, highly specific GFP- or RFP-binding proteins covalently coupled to the superior fluorescent dyes from ATTO-TEC. With our Booster you can: • Reactivate • Boost • Stabilize the signals of your fusion proteinsDirectly stain your protein of interest in fixed cells and benefit from the high specificity an small size that are perfect for su…

0.0/5.0
|0 Reviews

Chromobodies®

Chromotek GmbH

Chromobodies® - Your perfect reagents for cellular research including High-Content Analysis. Chromobodies are fluorescent nanoprobes suitable for real time analyses of endogenous cellular structures and processes in live cells. Chromobodies® are a novel species of extremely small intracellular antibodies. They are based on the antigen binding domain (VHH) derived from heavy chain antibodies of camelids genetically fused to a f…

0.0/5.0
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Epigenetic Toolbox

Chromotek GmbH

DNA methylation was the first epigenetic modification identified and has been intensively studied for more than half a century. By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development. However, the molecular mechanisms that ultimately…

0.0/5.0
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HTRF Assay

PerkinElmer

HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF has been proven in a variety of applications including GPCRs, kinases, epigenetics, PPIs, and the quantification of a wide range of biomarkers including cytokines.

0.0/5.0
|0 Reviews