Protein-Protein Interaction Assays Products & Reviews

The Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer is powered by the synergy of a high-resolution quadrupole mass filter, Thermo Scientific™ Orbitrap™ analyzer and novel Thermo Scientific™ Astral™ analyzer to achieve unsurpassed performance and experimental flexibility. The combination of the three mass analyzers enables the rapid acquisition of exceptional quality high resolution accurate mass (HRAM) full scan and MS…

Absolute characterization of protein-protein and other macromolecular interactions with composition-gradient multi-angle light scattering.

NanoLuc® Binary Technology (NanoBiT) is a two-subunit system based on NanoLuc® luciferase that can be applied to the intracellular detection of protein:protein interactions (PPIs) in live cells. The NanoBiT® system is composed of two small subunits, Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide), that are expressed as fusions to target proteins of interest. The LgBiT and SmBiT subunits have been independ…

The NanoBRET™ Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. This target engagement assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells

High-throughput sialylation screening for expediting the clone selection process

BRET-Based Assays to Detect Protein:Protein Interactions in Live Cells

Recombinant antigens and lentivirus for emerging SARS-CoV-2 variants

The Mammalian Two-Hybrid Assay Kit is a powerful method for detecting protein-protein interactions in vivo in mammalian cells. The two-hybrid assay kit utilizes hybrid genes to detect protein-protein interactions via the activation of reporter-gene expression.  Detects protein-protein interactions in mammalian cells  Works well in a variety of mammalian cell lines  Confirms suspected interactions between two proteins or i…

An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…

This guideline describes the design, development, and validation of quantitative assays for proteins and peptides by mass spectrometry.