ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
ACQUITY UPLC® HSS (High Strength Silica) T3 Columns contain the first and only 100% silica particle designed, tested and intended for use in applications up to 15000 psi/1000 bar. The ACQUITY UPLC® HSS particle is not an HPLC particle. High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high column packing and operating pressures of UPLC® separations. The first ligand chemistry in…
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Esay to take care, good and reliable even with harsh phases
Separation
Durable, reliable and longlisting. Even when using harsh mobile phases this can last more than 3000 injections
Review Date: 10 Feb 2023 | Waters
An excellent tool for analyzing a wide variety of molecules.
Analysis of secondary metabolites of medicinal plants
The column is very useful to analyze secondary metabolites of plants, as well as synthesis molecules, mycotoxins, among others. It is easy to create methods and mass chromatograms and spectra come out in good resolution.
Review Date: 27 Sept 2022 | Waters
Great results and easy to use
Quantitative and qualitative detection of plant secondary metabolites
The column is easy and can analyze many products, polar and non polar.
Review Date: 16 Dec 2021 | Waters
A basic column type for polar metabolites
UPLC column
One of the most used column types in our lab. We use it for polar metabolites and various specimens. High quality. Very reproducible and efficient.
Review Date: 15 Nov 2021 | Waters
One of our mostly used columns
UPLC column
Our gold standard for polar metabolites in metabolomics LC-MS analysis. We base a number of protocols in this column. Might be more expensive than the competition but is high quality.
Review Date: 15 Nov 2021 | Waters
A great product worth buying and using. Time and money saver.
Analysis of metabolites in natural products
HSS T3 columns are one of the best columns for the separation of mixtures of analytes. Working with natural products and analyzing huge mixtures of compounds is such a tough job but HSS T3 made it easy. Saving time, the separation is now easy as well as cost effective.
Review Date: 30 Oct 2020 | Waters
Truly, I can say it's great and one of the best.
Quantification of phenolics/flavonoids & theanine in medicinal plants
Great resolution and separation achieved by this HSS T3 column. I was struggling in the method development of mixtures of phenolics/flavonoids and Theanine, but after using the HSS T3 column, many of the problems of separating the standard mixture of phenolics and flavonoids were solved, with greater separation from end to end baseline with excellent repeatability. A wide range of compouds are now quantified and separated by this column. Thanks to Waters Corporation. Looking for buy a new one in the next month.
Review Date: 24 Jan 2020 | Waters
Very good product
Analysis of pesticides and biopesticides in all different matrices
The column is reliable and easy to use. normally there are no clogs or leaking issues and it is very robust
Review Date: 5 Jul 2019 | Waters
Fairly good produt for beverage analysis.
Food and beverage
Good product for amino acid sepration and sweetner analysis.
Review Date: 26 Mar 2019 | Waters
Brilliant column!
Metabolic profiling of urine samples
We love this column. It is robust, reliable and durable. It is one of our favourite standard column to do high-throughput metabolomic profiling analysis.
Review Date: 31 May 2016 | Waters
ACQUITY UPLC® HSS (High Strength Silica) T3 Columns contain the first and only 100% silica particle designed, tested and intended for use in applications up to 15000 psi/1000 bar. The ACQUITY UPLC® HSS particle is not an HPLC particle. High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high column packing and operating pressures of UPLC® separations.
The first ligand chemistry in this UPLC®-certified column family utilizes T3 bonding in order to retain and separate polar organic compounds. ACQUITY UPLC® HSS T3 columns possess the superior polar-compound retention, aqueous mobile phase compatibility and ultra-low MS bleed of Atlantis® T3 columns.
Easier Method Transfer
Since Atlantis® T3 HPLC and ACQUITY UPLC® HSS T3 columns utilize T3 bonding, LC methods can be transferred from HPLC to UPLC® separations. Because Atlantis® and HSS particles are different substrates, some method modification and/or optimization may be required. A compelling benefit of UPLC® technology is the ease and speed at which this method optimization can occur since UPLC® separations offer greater resolution in less time.
Moving from XBridge™ HPLC to ACQUITY UPLC® BEH columns is nearly seamless, as the hybrid particle substrates differ only in particle size. However, because of T3 bonding it should be straightforward to make simple adjustments to successfully transfer a separation from an Atlantis® T3 HPLC column to an ACQUITY UPLC® HSS T3 column.
