The MILLIPORE Re-Blot Plus Western Blot Strong Antibody Stripping Solution contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins. Advantages: No pungent-smelling b-mercaptoethanol is contained in the Antibody Stripping Solution. Antibody stripping is done at room temperature. No heating of blots is required. Blo…
Application Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples. Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available. Visit our Duolink® PLA Resource Center for information on how to ru…
6 mL vial of 2x concentrated Laemmli buffer for denaturing and loading of protein samples in SDS-PAGE.
Combining microscopy and flow cytometry, Amnis imaging flow cytometry systems provide bright-field, dark-field (SSC), and several fluorescent images of each cell in flow, at high speed. Paired with IDEAS® software for quantitative image analysis and population statistics, the technology gives you the ability to address complex biology that traditional flow cytometers can't resolve. Not only do the instruments reveal spatial lo…
A sesquiterpene alcohol found in many essential oils. Application trans,trans-Farnesol has been used in the synthesis of: • Cecropia juvenile hormone • terpenes, such as squalenes, cembranoids and forskolin
GenElute Agarose Spin Columns allow for the purification of linear DNA from agarose gels. The typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. The recovery decreases as the fragment size increases.
Cell Line Origin Mouse BALB/c monocyte macrophage Cell Line Description Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme. Application J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycoside…
Immobilon ® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.
For fast, reproducible and gentle extraction of active proteins from yeast and plant cells, use YeastBuster™ Protein Extraction Reagent. This reagent avoids the harsh conditions of vigorous mechanical or chemical treatment that often result in degradation of target proteins. Harvest cells and resuspend in YeastBuster™ protein extraction reagent, incubate for 15 minutes and then remove cell debris by centrifugation. Your extra…
BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.
Non-sterile goat serum collected from normal healthy goats. Used for blocking of interfering antibodies.
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria.
The Cell Proliferation Kit I (MTT) is a colorimetric assay for the non-radioactive quantification of cellular proliferation, viability, and cytotoxicity.
For molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Sterility testing culture media and rinsing fluids are critical components of your Steritest™ solution. They provide the highest level of quality and testing confidence, they have been formulated and tested to meet the requirements of the USP <71>, EU Pharmacopoeia < 2.6.1> and JP Pharmacopoeia <4.06>. Steritest™ sterility culture media and rinsing solutions are manufactured in an ISO 9001, environmentally controlled productio…
