UHPLC and HPLC Products & Reviews

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High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Scientific Surveyor Plus HPLC System

Thermo Fisher Scientific

The Surveyor Plus family of HPLC instruments is specifically optimized for the most demanding HPLC and LC/MS applications. Short cycle times, high sample capacity, low flow rate performance, and patented LightPipeTM technology have been integrated into one complete system for high-throughput sample processing environments. The versatility of the modular design, featuring custom quaternary pumping solutions, provides flexibili…

4.3/5.0
|7 Reviews

1290 Infinity LC Injector HTC/HTS

Agilent Technologies

High Throughput with Lowest Carryover      The Agilent 1290 Infinity LC Injector HTC/HTS is designed specifically to meet the throughput and reliability demands of critical LC/MS applications, offering lowest carryover with dynamic load and wash (DLW) principle. This highly versatile system is optimized for high throughput injections, and perfectly complements the infinitely better speed, resolution and sensitivity of the Agil…

4.3/5.0
|1 Review

Agilent 1220 Infinity II LC System

Agilent Technologies

The 1220 Infinity II LC is an integrated system for routine HPLC analysis in analytical and QA/QC labs. Based on the proven technology of the InfinityLab LC Series, the system's high quality and performance help you achieve maximum return on investment and lowest cost of ownership from day one.

4.4/5.0
|39 Reviews
  • Best New Separations Product of the Year

Nexera HPLC System

Shimadzu's Nexera is a genuine all-around HPLC that enables various types of analysis including conventional LC, ultra fast LC and ultra high-pressure LC up to 130 MPa without compromising performance and data quality. The LC-30A Nexera delivers high-speed and high-resolution analysis without sacrificing basic performance parameters such as excellent precision, near-zero carryover, good linearity, stability and ruggedness. O…

4.4/5.0
|20 Reviews

Thermo Scientific™ Vanquish™ Charged Aerosol Detector H

Thermo Fisher Scientific

Utilize the increased separation speed and resolution of the Thermo Scientific™ Vanquish™ UHPLC system to detect and quantify virtually any substance using the near-universal detection capabilities of Vanquish Charged Aerosol Detectors. The Thermo Scientific™ Vanquish™ Charged Aerosol Detector H provides enhanced linear dynamic range and accurate, consistent response to support research and method development. This detector ca…

2.7/5.0
|1 Review
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Allure AK Columns

Restek Corp.

This highly retentive, highly selective phase—unique to Restek—was developed specifically for the analysis of aldehydes and ketones as DNPH derivatives. Allure AK is a reversed-phase HPLC material that has the unique ability to separate all 13 carbonyl compounds specified in California Air Resources Board (CARB) Method #1004 using a simple acetonitrile/water gradient. Other columns require long analysis times or the use of tet…

3.0/5.0
|1 Review
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LenS3 MALS and MALS-V Multi-Angle Light Scattering Detector Series

Tosoh Bioscience

The LenS3 MALS detector offers a revolutionary approach for the measurement of molecular weight (MW) and radius of gyration (Rg) of synthetic polymers, polysaccharides, proteins, and biopolymers. A novel optical design, a unique cell-block assembly, and a new calculation methodology make the LenS3 MALS detector the first groundbreaking innovation in the light scattering technology arena in decades!

3.3/5.0
|1 Review
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Hitachi L-8900 Amino Acid Analyzer

Hitachi High Technologies America, Inc.

Hitachi has manufactured over 1800 Amino Acid Analyzers for over 40 years. The L-8900 is capable of analyzing physiological fluids and protein hydrolysates. If you have special requirements, the L-8900 is capable of a broad range of applications including clinical, food and pharmaceuticals. The L-8900’s unique post-column delivery design mixes the two ninhydrin reagents online just prior to amino acid derivatization which mean…

3.3/5.0
|1 Review
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LiChrosolv® UHPLC Solvents

Merck

For perfect high-performance liquid chromatography (HPLC) results, high-performance solvents are a must. LiChrosolv ® solvents are designed to optimally support analytical HPLC, fast chromatography, and LC-MS applications. They feature high degrees of UV transmittance, low particle counts, low acidity and alkalinity, and low evaporation residue levels making them ideal for reproducible separations.

3.0/5.0
|1 Review
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ACQUITY UPLC® BEH HILIC Columns

Waters

ACQUITY UPLC® BEH HILIC columns contain the rugged 1.7 μm unbonded BEH particles and are designed to retain and separate very polar basic compounds.These unique columns are optimized and tested to produce efficient and reproducible separations under UPLC® HILIC conditions. ACQUITY UPLC BEH HILIC columns overcome a major weakness of HILIC stationary phases: chemical stability. The unbonded silica stationary phases often used in…

3.3/5.0
|5 Reviews


DFL-10 Fluorescence Detector

D-Star Instruments Inc.

The new D-Star Instruments DFL-10 HPLC Fluorescence detector provides the sensitivity and the specificity of fluorescence detection at a price consistent with most low cost UV detectors. Sensitivity is enhanced in the DFL-10 by the new analytical flowcell that employs a unique excitation and emission geometry, a completely closed optical compartment, and a thermally stabilized system. The DFL-10 can be configured with a choice…

3.0/5.0
|1 Review

Coulochem® III Electrochemical Detector for HPLC

ESA - A Dionex Company

Coulochem® III Electrochemical Detection Platform Both Coulometric and Amperometric detection Available with DC and/or Pulse/Scan modes (or both) Compatible with all HPLC columns Ideal for methods development or routine applications requiring high-sensitivity detection of a single or small group of analytes Sensitive, flexible and exceptional stabilty

3.2/5.0
|4 Reviews

ZORBAX CN

Agilent Technologies

For normal-phase chromatography, the ZORBAX product line offers a choice of bonded and non-bonded silica packings. The ZORBAX CN is a polar bonded-phase column packing used for normal or reversed-phase high performance liquid chromatography, and is less prone to fouling than silica. The ZORBAX CN can be used for separations of compounds ranging from low to high polarity and is often utilized to separate compounds that have be…

3.3/5.0
|1 Review

Silica Gels for Chromatography – SiliaFlash®

SiliCycle Inc.

Synthetic silica, composed primarily of silicon dioxide, has become indispensable in today’s world in regard to its exceptional chemical stability. Consequently, uncountable commercial applications are now relying on synthetic silica, for instance, it is the most widely used stationary phase for chromatography. For nearly two decades, SiliCycle has been exploring silica’s great possibilities and versatility in order to chall…

3.3/5.0
|1 Review


Hypersil APS-2 Columns

Thermo Fisher Scientific

Hypersil APS-2 columns can be used with traditional buffers and organic additives as a weak anion exchanger for the analysis of anions and organic acids. Product detail: Hypersil APS-2 columns are a versatile aminopropyl group stationary phase which can be used in 3 modes: ion chromatography (weak anion exchanger), reversed phase and normal phase chromatography. Hypersil APS-2 columns can be used with traditional buffers a…

3.0/5.0
|1 Review