Enzymes Products & Reviews

Recombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

Recombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

MedixMDx Fast Bst Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature.  

MedixMDx Fast Bst RT Mix contains a Bst DNA polymerase that displays high strand displacement activity, and an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. MedixMDx Fast Bst RT Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature.  

MedixMDx qPCR Lyo ready mix contains optimized excipients, buffer, PCR enhancers, and an antibody-regulated hot-start Taq DNA polymerase and is ready to be lyophilized to produce stable reagents at room temperature. Upon addition of target-specific primers/probes to the master mix, the mixture can be lyophilized directly, without the need to add additional excipients.   

 MedixMDx qRT-PCR Lyo ready mix is ready to be lyophilized to produce stable reagents at room temperature. Upon addition of target specific primers/probes and reverse transcriptase (RTase Lyo) to the mastermix, the mixture can be lyophilized directly, without the need to add additional excipients.  

The Transcreener dAMP Exonuclease Assay directly measures dAMP produced by exonuclease enzymes as they cleave polynucleotides. 

Effectively removes and deactivates nuclease contamination even at high concentrations, safe on all laboratory glass and plastic ware, stable at room temperature for at least two years.

Cocktail Tablets supplied in glass vials Application Used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts in large volumes. Benefits Easy to use. Simply drop a quick-dissolving tablet into your buffer. Complete protection. Instantly protect your proteins against a broad range of proteases. Flexible. Performs in extracts from almost any tissue or cell…

Protease Inhibitor Cocktail Tablets supplied in EASYpacks Application Used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts in small volumes. Benefits Simplify protection. Proteins are protected against a multitude of proteases by simply adding a Complete Tablet to aqueous protein isolation buffer. Use a convenient tablet. Tablets combining different pr…

Protease Inhibitor Cocktail Tablets supplied in EASYpacks Application Used for the inhibition of proteolytic activity in small volumes in which EDTA may interfere with protein stability or subsequent assays or purification methods (e.g., Immobilized Metal Chelate Affinity Chromatography [IMAC]). Benefits Simplify protection. Proteins are protected against a multitude of proteases by simply adding a Complete Tablet to aqueo…

Application Dispase is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid and effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwante…

Application Dispase is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid and effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwante…

Phosphatase Inhibitor Cocktail Tablets supplied in EASYpacks Application When isolating or detecting phosphorylated proteins, benefit from the ultimate in convenience to protect your proteins against phosphatase activity. PhosSTOP Phosphatase Inhibitor Cocktail Tablets eliminate the time-consuming search for just the right phosphatase inhibitor mix. PhosSTOP is a proprietary blend of phosphatase inhibitors, formulated as a rea…

New recombinant Alkaline PhosphataserAPid Alkaline Phosphatase is used to dephosphorylate 5' ends of DNA and RNA. Alkaline phosphatase treatment prevents self-ligation of fragments by removing the 5'-phosphoryl termini required by ligases; this feature is of major importance in cloning strategies to decrease vector background. rAPid Alkaline Phosphatase can also be employed to dephosphorylate proteins. Use rAPid Alkaline Phosp…

Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments) for the following applications: Synthesis of first-strand cDNA for use in subsequent amplification reactions RT-PCR of GC-rich RNA templates Cy3, Cy5, DIG, biotin, and a…

Pwo SuperYield DNA Polymerase is ideal for high-fidelity amplification of DNA up to 3 kb. Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. This new buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of the amplification reaction with 18-fold increased fidelity compared to Taq DNA Polymerase. High Fidelity PCR…

Taq DNA Polymerase, 1 U/µl can be used instead of the higher concentration of Taq DNA Polymerase for all applications described. The supplied concentration allows accurate and convenient pipetting of small amounts of the polymerase. Benefit Obtain reliable, reproducible results. Roche Applied Science’s Taq DNA Polymerase exemplifies lot-to-lot consistency – tube-to-tube, experiment-to-experiment. Eliminate testing of each…

Thermostable DyNAzyme™ I DNA Polymerase is isolated and purified from Thermus brockianus, which is a Finnzymes proprietary strain. DyNAzyme I DNA Polymerase is more thermostable and tolerates DMSO better than Taq based polymerases.DyNAzyme I DNA Polymerase has a half-life of 3 hours at 96 °C. DyNAzyme I DNA Polymerase contains 5’-->3’ exconuclease activity but lacks 3'-->5' proofreading activity. DyNAzyme I DNA Polymerase is p…

Thermostable DyNAzyme™ II DNA Polymerase is isolated and purified from E.coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus which is a Finnzymes proprietary strain. DyNAzyme II DNA Polymerase is more thermostable and tolerates DMSO better than Taq based polymerases. DyNAzyme II DNA Polymerase has a half-life of 2.5 hours at 96 °C. DNA Polymerase contains 5’-->3’ exconuclease ac…

DyNAzyme™ EXT DNA Polymerase is an optimized mixture of DyNAzyme™ II DNA Polymerase and a proofreading enzyme. It is a versatile and easy-to-use enzyme especially suited for handling demanding reaction conditions without time-consuming optimization. DyNAzyme EXT Polymerease offers exceptional performance that Taq based mixtures cannot match.

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I. Highlights • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers A…

Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity. Highlights • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment (see Reference1) • Active in Therm…

Thermo Scientific T4 Polynucleotide Kinase (PNK) is a tetrameric protein composed of four identical subunits. It is widely used in molecular biology to radiolabel nucleic acids and to add a phosphate to the 5'-end of dephosphorylated nucleic acids to facilitate ligations. Features • Phosphorylates 5’ ends • Transfers 32P to DNA and RNA

Thermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.