Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector. In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed. In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…
The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.
The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.
Method completed in less than one day versus 2 days with restriction enzymes
The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).
The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.
Our Vitality hrGFP (humanized, Renilla reniformis green fluorescent protein) products are optimal for mammalian expression protein studies that examine gene expression, transfection efficiency, and protein localization. Since hrGFP is derived from a different organism than EGFP, the hrGFP antibody does not cross react with the Aequorea victoria GFP variant-derived EGFP.
Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.
The MycoSensor PCR & QPCR Assay Kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours.
