Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. New cDNA synt…
pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. CMV promoter for high-level protein expression in mammalian cells Large multiple cloning site contains 15 unique sites Clone PCR products generated with any PCR e…
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods.
The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells. In situ staining of cells directly in dishes without removing or manipulating cells Rapid detection of Ÿ-gal activity in transfected cells Results in 24 hours Maximum signal with low background Deep blue staining reaction Kit in…
Our single-stranded DNA binding standard is a helix-destabilizing E. coli protein. The 75.6-kD protein is essential for replication, recombination, and repair processes.
The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD). Detects protein-prote…
The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene. Tightly controlled T7 RNA polymerase-based gene expression in E.coli High-level expression upon induction Convenien…
The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene. Tightly controlled T7 RNA polymerase-based gene expression in E.coli High-level expression upon induction Convenien…
The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression. The establishment of a highly regulated expression system in eukaryotic cells is an invaluable tool for the study of cell cycle, oncogenicity, cytogenicity, and the mechanisms of gene regulation.
The Luciferase Assay Kit provides rapid, sensitive, and quantitative measurement of American firefly (Photinus pyralis) luciferase activity in cultured mammalian cells. Sensitive, widely used reporter assay Rapid, quantitative results Reporter activity immediately available upon translation Useful in conjunction with PathDetect systems
The LightSwitch promoter reporter GoClone collection includes transfection-ready luciferase reporter constructs utilizing the novel RenSP luciferase technology for over 17,000 human promoters and highly optimized LightSwitch reagents. Measure the functional response of promoters to stimulus in nearly any cell-based system. Plus, advances in gene assay reagent and transfection technology eliminates the need for co-transfection…
An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…
A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…
APAgene™ GOLD Genome Walking Kits are designed to rapidly and reliably amplify unknown genomic DNA using our patented APA technology: 1. Isolate unknown genomic sequences flanking transgenes (including T-DNAs, gene traps, transposons), sequence-tagged sites (STSs) or expressed sequence tags (ESTs). 2. Isolate localized sequences flanking known sequences from large clones and genomic DNAs. 3. Sequence insert ends of large clone…
Improve your cell analysis and use our powerful new transfection reagents: Efficiently transfect a broad spectrum of cells, including difficult-to-transfect cell lines and tumor cell lines. Generate physiologically relevant results using low cytotoxic reagents. Avoid time-consuming optimization using fast, reliable protocols.
OneTRAC™ allows you to profile every gene, validate biomarker signatures, and apply your findings across all your studies. One-stop service for all your gene expression profiling needs All-inclusive gene expression data for pattern recognition and pathway analyses at the macro- and micro-levels Streamlined and integrated methodology from screening to validation of gene signatures Cost-efficient expression profiling f…
GeneJuice® Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity. This transfection reagent is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. Whereas many available transfection reagents…
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Based on the T7 promoter-driven system, Novagen’s pET System has been used to express thousands of different proteins. pET Vectors are supplied as purified plasmid DNA (10 µg) plus an Induction Control strain (supplied as a glycerol stock). Complete sequences and detailed vector maps are available.
Powerful transfection reagent, based on an innovative biochemical method. Next generation of outstanding transfection reagent, highly efficient, universal and multipurpose.
For all nucleic acids and all transfection conditions.The universal and powerful PolyMag formulation allows achieving very high transfection efficiency. It is composed by magnetic nanoparticles coated with cationic molecules. Exclusive and optimized PolyMag reagent has been successfully tested with:- All nucleic acids types: naked DNA, RNA, siRNA, ODN, etc…- Cell lines, primary and hard to transfect cells- Adherent and suspens…
CombiMag improves your transfection reagent efficiencyCombiMag reagent has been designed to be employed in association with all transfection reagents complexed with any types of nucleic acids. All cell types, even hard-to-transfect and primary cells, can be transfected using CombiMag associated to any working reagent, even if its original efficiency is low.CombiMag has been associated with many existing transfection reagents i…
