Gene Expression and Molecular Cloning Products & Reviews

An optimized and streamlined protocol for high-resolution genome-wide mapping of transcription factor binding sites in the human and mouse genomes

Providing the highest quality ChIP-enriched DNA for analysis by qPCR or Next Generation sequencing

Specially formulated to perform ChIP from PBMCs, including lymphocytes (T cells, B cells and NK cells), monocytes and dendritic cells

Modified ChIP assay that utilizes the CRISPR/Cas9 system to target a specific DNA locus for immunoprecipitation

Perform ChIP without the need for protein-specific antibodies

Detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation for analysis

Immunoprecipitate and enrich for DNA fragments containing 5-hydroxymethylcytosine (5-hmC)

Immunoprecipitate and enrich for single-stranded DNA fragments containing 5-methylcytosine (5-mC)

Fast magnetic assay capable of efficiently isolating methylated CpG islands from fragmented genomic DNA

Novel technology that allows for positive identification of unmethylated DNA fragments

Reagents to measure luciferase reporter signal as part of the LightSwitch Luciferase Assay System

Custom cloning services to clone any fragment from the human, mouse or rat genome into any LightSwitch luciferase reporter vector

Custom site-directed mutagenesis services to create any sequence variant from any existing LightSwitch reporter vector

Cell-based screening service to measure the effects of compounds on a variety of biological pathways

Sequence Variant Assay Services to understand the functional impact of sequence variation on promoter or 3´UTR activity

Full screening service to validate the 3´UTR targets of specific miRNAs

Adenoviral transduction enhancer.

The DriverMap™ Human Genome-Wide Targeted Expression Profiling Assay enables researchers to simultaneously measure the expression level of almost 19,000 human protein-coding genes in a single assay. By combining highly multiplexed RT-PCR amplification with the depth and precision of next-generation sequencing (NGS) quantitation, the Driver-Map assay provides convenient, comprehensive, highly sensitive, and quantitative meas…

Want to know if Cas9 is active in your cells? Cellecta’s CRISPRtest™ method allows you to assess the level of Cas9 activity in your cell line using two pre-mixed lentiviral-packaged vectors, the first containing a green fluorescent protein (GFP) marker and gRNA sequence targeting an essential gene (PCNA), and the second containing a red fluorescent protein (RFP) marker and a non-targeting gRNA. Transduction of CRI…

Cellecta’s Ready-to-Use Lentiviral Packaging Plasmid Mix allows the production of pseudoviral particles when mixed with second- or third-generation lentiviral vectors. The Packaging Plasmid Mix is a mixture of the human immunodeficiency virus (HIV) lentiviral packaging plasmid psPAX2 and the pMD2.G plasmid containing VSV-G. These plasmids provide all the necessary structural, regulatory, and replication proteins required to…

Highly accurate RNA quantification with single cells or low-input bulk RNA

Perfect for: Real-time fluorescent RNA detection, facilitating use of different RPA reaction volumes, or variation of component ratios.

Perfect for: Real-time fluorescent DNA detection, facilitating use of different RPA reaction volumes, or variation of component ratios

Full phenotypic analysis  to connect genes to behavior

Animal models engineered for human disease discovery