Enzymes Products & Reviews

Catalyzes hydrolysis of amide linkages

T4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme. Both blunt and cohesive end DNA ligation, as well as single-stranded nick repair of DNA, RNA, and DNA/RNA, are possible using the T4 DNA ligase.

Detect microbial deoxyribonuclease enzymes, particularly from staphylococci, with Thermo Scientific™ Oxoid™ DNASE Agar (Dehydrated).

MegaFi™ Fidelity DNA Polymerase is the best high fidelity DNA Polymerase on the market. With 1300X better fidelity than regular Taq, this ultrasensitive polymerase amplifies even the most difficult templates.

DNA specific exonuclease Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5' to 3' direction Highly processive degradation of double-stranded DNA from the 5' end Preferred substrate is 5'-phosphorylated double-stranded DNA although non-phosphorylated substrates are degraded at a greatly reduced rate Lambda Exonuclease is ideal for: Conversion of linear double-stranded DN…

Super. Fast. PerfeCTa.

UV, Hexokinase

BbsI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10119525.  NEB is excited to announce that all reaction buffers are now BSA-free. NEB began switching their BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

Simplify your research with reliable products that "Keep it easy!". Save time, expense, and handling steps while increasing convenience and maximizing success. Make protease inhibition even easier with: New cOmplete EASYpacks: Rely on the protection you've always obtained with cmplete Tablets - now in even more convenient packaging. New cOmplete Lysis kits: Choose the single product to combine simple, rapid lysis of bact…

Protease Inhibitor Cocktail Tablets supplied in glass vials Application Used for the inhibition of proteolytic activity in large volumes in which EDTA may interfere with protein stability or subsequent assays or purification methods (e.g., Immobilized Metal Chelate Affinity Chromatography [IMAC]). Benefits Easy to use. Simply drop a quick-dissolving tablet into your buffer. Complete protection. Instantly protect your pro…

Thermo Scientific RNAse Inhibitor specifically inhibits some of the most common types of RNases making it the perfect solution to protect your RNA. Thermo Scientific RNase Inhibitor is isolated by affinity chromatography from porcine livers. This enzyme acts on type A RNases (RNase A, B and C) and functions in any enzymatic reaction buffer with pH 5-8 (optimal activity is observed at pH 7-8). Porcine RNase Inhibitor does NOT i…

Thermo Scientific rDNase I is produced through a process that contains no animal by-products. The enzyme is provided free from any detectable RNase activity or animal derivatives. rDNase I has a molecular weight of approximately 30kDa. As an endodeoxyribonuclease, DNase I hydrolyses phosphodiester linkages yielding di-, tri-, and oligonucleotides with a 5' phosphate and a free 3' hydroxyl. DNase I has been shown to act on sing…

Properties: Endoribonuclease that preferentially cleaves 3' to G. Guanosine-3'-phosphates and oligonucleotides with guanosine-3'-phosphate terminal groups are the final products. Substrate is single-stranded RNA. Source: Aspergillus oryzae Volume activity: approx. 105 U/ml Unit definition: One unit according to Egami releases a sufficient quantity of acid-soluble oligonucleotides to produce an absorbance increase of 1.0 at 260…

phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5´ proofreading exonuclease activity. Source: An E. coli strain that carries the phi29 DNA Polymerase gene from bacteriophage phi29 Applications: •Replication requiring a high degree of strand displacement and/…

FastDigest® restriction enzymes are an advanced line of restriction enzymes for rapid DNA digestion. All FastDigest® enzymes are 100% active in the universal FastDigest® Green buffer and are able to digest DNA in 5-15 min. This enables any combination of restriction enzymes to mork simultaneously in one reaction tube and eliminates the need for sequential digestions. FastDigest® enzymes can be used to digest plasmid, genomica…

Appropriate for COVID-19 detection.

100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

250 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl2

PCRBIO HS Taq Mix Red is an advanced antibody-mediated hot start DNA polymerase mix designed for fast, highly specific and sensitive PCR. The mix contains a red dye allowing reactions to be directly loaded onto agarose gels without additional loading buffer. Whether you need a hot start assay for high-throughput or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading 2x mix.

Eliminate the need for time-consuming DNA extraction methods with this simple, integrated extraction and amplification kit powered by the latest advances in hot start polymerase technology. PCRBIO Rapid Extract PCR Kit combines rapid DNA extraction with fast, highly specific DNA amplification in a convenient, easy-to-use format. Generate PCR-ready DNA in 15 minutes, without spin columns or hazardous chemicals.

Recombinant, high-purity endonucleases for genome editing experiments

Hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues (Asp and Cys)

Bioluminescent Assays to Detect NAD(P)+ and NAD(P)H Levels in Cells Homogeneous, single-reagent-addition assays Easily adapted for high throughput screening formats

Experience improved PCR performance with GoTaq® DNA Polymerase products. GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and in some cases superior to that of standard Taq DNA polymerase. GoTaq® DNA Polymerase comes in a variety of formulations designed to give you maximum flexibility, control and convenience.

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibit…