Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Brilliant II SYBR qPCR and QRT-PCR reagents provide improved sensitivity and detection while ensuring reproducible quantification at low target concentrations. Compared to other commercially available qPCR reagents, Brilliant II SYBR qPCR and QRT-PCR reagents have earlier threshold cycle (Ct) detection at high and low template concentrations.
Brilliant III qPCR and QRT-PCRE Master Mixes are more sensitive and faster via the quick-activating hot start and the engineered Taq-mutant. Brilliant III ultrafast maintains amplification efficiency, R2, dynamic range, and detection sensitivity. Shorter total cycle time.
Brilliant III Ultra-Fast SYBR® QPCR and QRT-PCR Master Mixes deliver superior specificity by utilizing a novel faster-activating hot start method to minimize the formation of primer-dimers and other off-target reactions.
Alien Reference RNA QRT-PCR Detection Kit for Monitoring the Overall Performance of QRT-PCR Assays. High-quality external control for detecting inhibitors in RNA samples Highly sensitive to various inhibitors No significant homology to any known sequences Ideally suited for assay standardization applications
Takara produce a host of products for PCR Takara’s new sensitive qPCR premixes contains TLi RNase H and are developed to address the inhibitory effect on the PCR due to the presence of remaining RNA after cDNA synthesis. PrimeSTAR®, a non-Taq polymerase with high proofreading activity are now available in a high speed version, PrimeSTAR Max, with extension rate of less than 5 sek/kb and PrimeSTAR GXL, that amplifies up to 30 k…
Fosmid clones are similar in size (ca. 40kb) to cosmids, but, like BAC clones, they contain replicons derived from the F factor for DNA replication and segregation. Because of this property, they are present in low copy number and consequently more stable than cosmids. Also, the copy control vector can be used for easy DNA purification from fosmid clones. Fosmid libraries are commonly used for closing gaps in a whole genome se…
The miScript miRNA PCR Arrays accurately analyze the expression of multiple microRNA (miRNA) sequences at the same time on ANY real-time PCR instrument. This new generation of miRNA PCR Array builds on the original strengths of the RT² miRNA PCR Arrays* with new pathway content, expanded assay coverage plus added species all with the flexibility and sample preparation advantages of the miScript PCR System. The available 96-…
Mx3000P & Mx3005P plates, tubes, filter sets, and replacement lamp assemblies.96-well QPCR Plates, 8-Strip Tubes, Single Tubes, and Optically Clear Caps Benchtop Racks for QPCR Tubes Replacement Lamp Assembly
The Applied Biosystems 7300 Real-Time PCR System gives you advanced, multicolor real-time PCR capabilities with exceptional budget sensitivity. Run time of less than 2 hours Four-color detection enables you to perform gene expression analysis, pathogen quantitation, SNP genotyping, and plus/minus assays with internal positive controls Study gene expression, detect and quantitate pathogens, perform allelic discrimination…
Robotic workstation for fully automated nucleic acid preparation and filling of LightCycler® Capillaries, 96-well PCR plates, and tubes suitable for the most commonly used PCR instruments Benefits Save time with rapid, fully automated isolation of high-quality DNA, RNA, and mRNA. Obtain reliable, reproducible results. Benefit from high flexibility in sample type and volume, as well as elution volume and post-elution p…
TaqMan® Gene Expression Assays are a comprehensive collection of over 1,000,000 probe and primer sets for quantitative gene expression analysis of 13 species including human, mouse, rat, Arabidopsis, Drosophila, and C. elegans genes. These assays let you quickly, easily, and affordably perform precise gene expression studies using real-time PCR. TaqMan®-based assays provide unmatched sensitivity, specificity, and reproducibi…
With over 4.5 million SNP assays available, including 3.5 million HapMap SNPs, 70K cSNPs, and 160,000 validated assays, Applied Biosystems SNP Genotyping Assays make it easy to perform SNP genotyping studies with the precision of TaqMan® reagent-based chemistry. These assays provide SNP detection capabilities with high-confidence results. The large selection of SNP assays helps advance your screening, association, candidate…
Applied Biosystems GeneAmp® Fast PCR Master Mix's sensitive, pre-mixed hot-start chemistries reduce PCR reaction time to as little as 25 minutes when used with the 9800 Fast PCR System. • Pre-mixed hot-start chemistry enables you to set up your experiments quickly. • Detect a single copy of a gene in as little as 10 ng of DNA. • Get reproducible, specific, and sensitive results.
Extensor Long Range Enzyme Blend is an optimized mix of ThermoPrime* DNA Polymerase and a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate long PCR products, up to 20kb. This makes the enzyme blend ideal for robust full-length amplification of long range sequences. ■ Amplifies targets up to 20kb in length ■ Unique enzyme blend and optimized buffer combination delivers increased accu…
Introducing the LightCycler® 96 Real-Time PCR System What if you had the power to get your qPCR data fast, reliably and with less effort? Well, now you can. The result of more than a decade of Roche experience developing high-performance qPCR systems is now accessible for any research lab. The LightCycler® 96 System delivers data that makes you a publication superhero: • Fast precision thermal cycling • Leading-edge optics fo…
The TaqMan® One-Step RT-PCR Master Mix Reagents Kit, which combines the reverse transcription and amplification steps, permits excellent results even when quantifying minute amounts of RNA. Set-up is quick, easy, and minimizes risk of sample crossover and contamination, making this kit well-suited for all high-throughput applications. • Optimized for high sensitivity and yield • Combined RT (reverse transcription) and amplific…
Syzygy FlashTaq 2x Master Mix is a ready-to-use 2x mixture of FlashTaq, dNTPs, buffers and magnesium for efficient PCR amplification. Just add template DNA, primers, and water. FlashTaq Hot-Start DNA Polymerase is a chemically modified Taq polymerase designed for reducing nonspecific DNA amplification including primer dimers. The activiation time for FlashTaq is only 2 minutes at 95°C, which is faster than that for leading a…
rEVAlution 2X qPCR Master Mix is a ready to use hot-start qPCR master mix with EvaGreen® and FlashTaq, a hot start Taq polymerase. A 25μM ROX reference dye is supplied separately for use with some real time instruments. The master mix is optimized to work with both fast cycling conditions and regular three step cycling conditions. Available in 3 pack sizes: 100 20µL Reactions, 500 20µl reactions and 1,000 20µl reactions.
PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR. Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension,…
Rapid and accurate detection of foodborne pathogens
Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…
SensiFAST™ cDNA Synthesis Kit has been developed to generate high-quality first strand cDNA from total RNA template. SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first strand cDNA synthesis for use in real-time PCR studies (fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, reg…
