PCR and Thermal Cycling Products & Reviews

Rapid and accurate detection of foodborne pathogens

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

Obtain high quality results with this thermostable probe-based qPCR master mix

1 ml, one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 100 x 20 µl reactions

SensiFAST™ cDNA Synthesis Kit has been developed to generate high-quality first strand cDNA from total RNA template. SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first strand cDNA synthesis for use in real-time PCR studies (fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, reg…

SensiFAST™ Probe Hi-ROX Kit and SYBR® Hi-ROX Kit have been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Kits delivers fast, highly-specific and ultra-sensitive real-time PCR.SensiFAST™ Probe Hi-ROX Kit has been d…

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions

Our comprehensive UltraFlux® PCR offering includes tubes, strips, plates, and sealing film for any experimental need. UltraFlux Tube and Strip Tube Features: Angularly attached caps on the UltraFlux® i and SnapStrip® strip tubes prevent cap lids and hinges from interfering with each other. Ultra-thin walls ensure efficient thermal transfer and thus maximum yield. SSI wall thickness is 0.20mm compared to thicker 0.25mm of…

Verigene® System instrumentation consists of a Verigene Reader and one or multiple Verigene Processor SPs. The Verigene Processor SP is a modular, easy-to-use benchtop analyzer that combines automated nucleic acid extraction, purification, amplification (if required) and hybridization in each module. These advanced capabilities allow users to run Verigene Tests on-demand in response to testing needs, without the need for batc…

SapphireAmp Fast PCR Master Mix is a hot start PCR master mix that contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a convenient 2X premix. SapphireAmp PCR Master Mix is especially designed for fast PCR reactions that can be completed in half the time required when using conventional Taq DNA polymerase. For human genomic DNA, amplification of 2 kb targets can be…

PrimeSTAR GXL DNA Polymerase is a high fidelity polymerase that provides efficient PCR amplification even in challenging situations, such as when amplifiying GC-rich templates, when there is eXcess template in the reaction mixture, and when amplifying Long products up to 30 kb (GXL). PrimeSTAR GXL Polymerase is the most robust high fidelity polymerase available, routinely allowing amplification of PCR products that cannot be…

The Takara Bio TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies.

SingleShot™ Probes One-Step Cell Lysis and RT-qPCR Analysis Kit, 100 x 50 µl reactions

Made of thin wall polypropylene and designed for precise fit in heat blocks to optimize heat transfer Nonsterile and certified DNase- and RNase-free Autoclavable at 121°C and will withstand centrifugation to 10,000 x g

Axygen® microplate sealing films are designed for applications ranging from PCR and Real-Time PCR to ELISA and cell culture. Axygen’s sealing films are the easiest and most cost-effective method for sealing plates. They can be used in wide temperature ranges, making them suitable for many applications.Allows effective gas exchange for cellular and bacterial cultivation while preventing contaminationSeal polypropylene and polys…

Axygen® microplate sealing films are designed for applications ranging from PCR and Real-Time PCR to ELISA and cell culture. Axygen’s sealing films are the easiest and most cost-effective method for sealing plates. They can be used in wide temperature ranges, making them suitable for many applications.Provides exceptional seal on multiple well plates with a wide range of reagents and temperaturesWhen used in conjunction with A…

The new MaxyGene II Thermal Cycler replaces our successful and reliable MaxyGene. It brings increased speed and advanced features, providing the premium performance you have come to expect from Axygen brand products.Unique flexible programmingRapid run timesImproved workflow over traditional gradient cyclersRamping rates up to 5°C/sec.Simple user interfaceAdjustable heated lid accommodates strips, tubes and microplatesPre-prog…

Axygen® 0.5 mL polypropylene thin wall PCR tubes are designed to fit standard 96 well blocks.RNase-/DNase-freeNonpyrogenicNonsterileManufactured with 99.9% pure virgin polypropyleneCompatible with ABI-310 InstrumentCaps for these tubes are AM-SEPTA-310 Axygen® AxyMats™ Single Septa Cap Compatible with ABI-310 Sequencer, Nonsterile

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ab…

100 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water

The world’s first Magnetic Induction Cycler.

Platinum® Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzymePyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology.   Features:…

The Femto™ Bacterial DNA Quantification Kit allows for quantification of as little 20 femtograms of bacterial DNA in as little as 1 µl of sample.

Fluent’s groundbreaking design delivers more capacity and productivity for genomic workflows. This innovative workstation offers superior precision, higher throughput and longer walkaway times, allowing scientists to get more done in less time, with greater confidence. The Fluent Laboratory Automation Workstation for genomic workflows offers high precision liquid handling – down to 200 nl – for accurate quantification se…

Assess bias and errors in NGS-based microbial composition profiling workflows