Cell Death / Health Assays
ApopTag® Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits
Rat mammary gland undergoes programmed cell death processes during the tissue regression that follows lactation. Apoptotic bodies, which can be seen by routine histologic staining, can be specifically stained by techniques that localize DNA strand breaks in situ. The specificity of staining for strand breaks can thus be correlated with the morphological changes that define the process of apoptosis.
APOPTAG® Plastic Coverslips are designed to be custom fitted to the specific APOPTAG® kit application.
The ApopTag® Peroxidase In Situ Oligo Ligation Kit specifically detects apoptosis by staining only cells that contain double-stranded breaks that are blunt-ended or have a one base 3' overhang (cells containing nicked, gapped, 3'-recessed, 3'-overhanging ends longer than one base and single-stranded ends are not detected). The high analytic specificity of the ApopTag® ISOL Kit minimizes background due to non-specific labeling…
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a s…
Chemicon's Quick Apoptotic DNA Ladder Detection Kit provides a fast and easy means for detection of DNA fragmentation in apoptotic cells. Unlike other similar kits which require 1-2 days to finish the procedure, the new detection method requires less than 90 minutes to prepare DNA in a single tube, without the need for extraction or the use of columns. DNA fragmentation can be easily visualized by agarose gel electrophoreses.…
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a s…
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-9 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the LEHD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate LEHD-pNA. The free pNA can be quantified using a spectropho…
The CHEMICON Glutathione Detection Kit is a quick method for detection of intracellular levels of reduced glutathione (GSH). Reduction in levels of GSH may be an early indicator of apoptosis.
MTT is a pale yellow substrate that is cleaved by living cells to yield a dark blue formazan product. This process requires active mitochondria, and even freshly dead cells do not cleave significant amounts of MTT. The colorimetric assay described below can be used for either proliferation or complement-mediated cytotoxicity assays.
CHEMICON®'s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation ca…
ToxCount™ provides a two wavelength cell viability assay based on determination of live and dead cells. The assay uses fluorescent dyes that measure permeability of the plasma membrane of dying cells and the ability of living cells to retain intracellular esterases, respectively. Live cells are distinguished by the presence of ubiquitous intracellular esterase activity, determined by the enzymatic conversion of the non fluores…
Comet Assay IV's unique single-click scoring method and ultra-fast live video picture make it the most efficient and easy-to-use system available for measuring DNA damage using single cell gel electrophoresis. There are no complicated parameters to adjust and no hardware to install - simply connect the camera and start scoring. Working closely with leading experts in the field, Perceptive Instruments have released Comet Assa…
The Thermo Scientific ToxInsight Drug Induced Liver Injury Assay Cartridge for the ToxInsight IVT Platform offers a new multi-parameter cell-based assay to determine the risk of liver injury by chemicals being tested for new drugs or for consumer products safety assessment. The ToxInsight DILI Cartridge is for identifying compound hepatotoxicity by simultaneous detection of multiplexed targets, including cell loss, nuclear DNA…
Abasic site quantification in genomic DNA. Determine the number of abasic sites in genomic DNA samples, colourimetric microplate assay, detection range: 1-40 abasic sites per 1x10^5 base pairs DNA.
Cellstain-Double Staining Kit is utilised for simultaneous fluorescence staining of viable and dead cells. Flurometric microscope detection, simultaneous determination of viable and dead cells.
Calbiochem® supplies a wide variety of products for the study of apoptosis, includingActivators and Inhibitors Antibodies and Blocking Peptides Assay Kits Caspase-related products Mitochondrial Permeability-Transition Reagents and Related Products Poly(ADP-Ribose) Glycohydrolase (PARG) Inhibitors Poly(ADP-Ribose) Polymerase (PARP) Inhibitors Telomerase Products View the online interactive pathways to easily locate the product…
The Multiparameter Apoptosis Kit comprises three spectrally distinct fluorophores that label cellular targets to allow assessment of cell health by looking at several biological parameters. This kit specifically labels nuclei, f-actin, and mitochondrial mass/potential in cells. The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system. Available in Evaluation Size (Five…
The Multiparameter Cytotoxicity 1 Kit comprises three spectrally distinct fluorophores that label cellular targets to allow assessment of cell health by looking at several biological parameters. This kit contains indicators for nuclei, permeabilized cells, and lysosome mass/pH. The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system. Available in Evaluation Size (Five…
Being able to visualize endogenous cellular structures in live cells is key for many high content analyses. The two Chromobodies® of the ChromoTek’s Cell Organelle Toolbox offer a new way to investigate key compartments of the cell non-invasively. Lamin-Chromobody® The new Lamin-Chromobody® is a major breakthrough especially in High-Content Analysis (HCA) as apoptosis assays thus far rely on static endpoint assays. The nucle…
The FlowCellect™ Human NK Cell Characterization Kit includes three directly conjugated antibodies, Anti-CD3-APC, Anti-CD56-PE, and Anti-CD16 FITC. This three color kit is designed to characterize NK within mixed cell populations. The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 three-colo…
DNAQual kit is used for the quantitative assessment of DNA degradation by quantifying the number of copies of a target gene compared to a calibrator gene (included in the kit) in any human DNA sample by qPCR in duplex in 90 minutes. The two genes (target and calibrator are measured with two specific probes carrying each a different fluorophore: Texas Red for the calibrator and VIC for the target gene. The software on the qPCR…
Assessment of Neuronal Activity Using Microelectrode Array. The eCiphrNeuro assay uses primary cultures of rat cortical neurons. Cyprotex’s neuronal assay uses high throughput microelectrode array (MEA) technology to monitor electrophysiological activity. Neurons grown on microelectrode arrays recapitulate many features of neurons in vivo, including spontaneous activity (spiking and bursting), plasticity, organisation a…
Detect caspase-1 activity with the FLICA® Caspase 1 Assay Kit. This in vitro assay employs the green fluorescent inhibitor probe FAM-YVAD-FMK to label active caspase 1 enzyme in living cells or tissue samples. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or by flow cytometry. Caspase 1 (ICE):Members of the mammalian caspase family of cysteinyl aspartate-specific proteases play disti…
Magic Red™ Caspase 3 and 7 Assay is a substrate-based fluorescent apoptosis assay that measures DEVDase enzyme activity in living, intact cells. Detection of caspase 3 and 7 activity (DEVDase enzymes) is a reliable method for assessing apoptotic activity in experimental cell populations. The Magic Red™ caspase 3 and 7 fluorogenic substrate, MR-(DEVD)2, is primarily targeted by caspases 3 and 7 of the apoptotic protease casca…
