Gene Expression and Molecular Cloning Products & Reviews

TransIT-TKO® is a broad spectrum siRNA transfection reagent that enables high efficiency siRNA delivery and knockdown of target gene expression in many cell types including primary cells.

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).   Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…

ViaFect™ Transfection Reagent allows high-efficiency transfection of a wide range of cell types, including iPS Cells, without compromising cell viability.   ViaFect™ Transfection Reagent enables the design of biologically relevant assays in workhorse adherent cell models, suspension cells important for studying cell signaling, and even differentiated cell lines derived from stem cells. ViaFect™ Transfection Reagent offer…

The new InjectMan® 4 is ideal for microinjection into adherent cells, smaller organisms and embryos in the early stages of development. The combination of InjectMan 4, FemtoJet® 4i or 4x enables a fast, semi-automatic injection. Furthermore, the InjectMan 4 is the ideal micromanipulator for all complex applications that require a dynamic movement mode and direct control of the injection process via the joystick button. The ax…

The MycoAlert™ PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlert™ PLUS Substrate, c…

Contains one vial of Lipofectamine® 3000 Reagent (0.75 mL) and one vial of P3000™ Enhancer Reagent.

Designed to allow high quality DNA enrichment from as little as 1000 cells per ChIP reaction for abundant targets

Active Motif offers FactorPath™ ChIP-Seq Services to map protein-DNA interactions & histone modifications

Making it possible to perform ChIP in just 1 day

Ideal for recombinant lentivirus production

NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, a…

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C ) Fast - 5 minutes for cohesive or blunt ends. Convenient - ligation performed at room temperature. Flexible - suitable for all common ligation reactions.

Ideal for Recombinant Adeno-associated Virus and Lentivirus Production

Genetic engineering is the process of manipulating the genetic material of an organism — often to include the DNA from a foreign organism. Using the classic pGLO Bacterial Transformation Kit, students transform bacteria by introducing a gene from the bioluminescent jellyfish Aequorea victoria. The same procedure has been used to create "designer proteins" which have led to the explosion of new health treatments, agricultural a…

Capped or Uncapped RNA Transcripts for in vitro or in vivo Translation

mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its s…

SHERLOCK is an evolution of CRISPR technology, which others use to make precise edits in genetic code. SHERLOCK can detect the unique genetic fingerprints of virtually any DNA or RNA sequence in any organism or pathogen. Developed by our founders and licensed exclusively from the Broad Institute, SHERLOCK is a method for single molecule detection of nucleic acid targets and stands for Specific High Sensitivity Enzymatic Report…

Recombinantly produced in E. coli, CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUTANA™ pAG-MNase contains a C-terminal 6xHis epitope tag. CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows Formulation: CUTANA™ pAG-MNase is provided as a 20…

Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Assembly and transformation in just under two hours Flexible sequence design (scar-less cloning) No PCR clean-up step required High transformation efficiencies for inserts up to 20 kb Easily adapted for multiple DNA manipulations, including site-directed mutagenesis

For seamless DNA cloning in a lyophilized format

High-efficiency guide RNAs for knockouts and knock-ins.

Gibson Assembly® allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.

Double-stranded DNA fragments for high-throughput screening applications

PD-L2/TCR Activator Mammalian Expression Kit

MOA-based functional bioassay to measure Fc effector activity.