Gene Expression and Molecular Cloning Products & Reviews

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Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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TransIT®-2020 Transfection Reagent

Mirus Bio LLC

A high performance, animal-free, broad spectrum DNA transfection reagent. Broad Spectrum DNA Delivery - Achieve high expression in many cell types, including hard to transfect cell lines and primary cells Outperforms Competitor Reagents - TransIT®-2020 demonstrated higher protein yield when compared to FuGENE® HD, Lipofectamine™ 2000, and Lipofectamine™ 2000 CD Superior Transfection of Insect Cells - Obtain higher expre…

4.7/5.0
|3 Reviews

Quik Change Site-Directed Mutagenesis

Agilent Technologies

The original QuikChange Site-Directed Mutagenesis Kits eliminate the need for subcloning into M13-based bacteriophage vectors and for ss-DNA rescue. This makes site-directed mutagenesis studies simple and reliable since it allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. 

4.7/5.0
|3 Reviews

TaqMan Gene Expression Assays

Thermo Fisher Scientific

TaqMan® Gene Expression Assays are a comprehensive collection of over 1,000,000 probe and primer sets for quantitative gene expression analysis of 13 species including human, mouse, rat, Arabidopsis, Drosophila, and C. elegans genes. These assays let you quickly, easily, and affordably perform precise gene expression studies using real-time PCR. TaqMan®-based assays provide unmatched sensitivity, specificity, and reproducibi…

4.7/5.0
|14 Reviews



Q5® Site-Directed Mutagenesis Kit

New England Biolabs Inc.

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

4.8/5.0
|4 Reviews

TransIT-X2™ Dynamic Delivery System

Mirus Bio LLC

An innovative, polymeric system for efficient delivery of plasmid DNA and siRNA/miRNA. Experience the transfection breakthrough. Achieve superior transfections with an innovative polymeric system that efficiently delivers both DNA and RNA out of the endosome and into the cytoplasm overcoming a critical barrier to nucleic acid delivery.Head-to-head comparisons against the Lipofectamine® 2000 illustrate superior or equal lucife…

5.0/5.0
|2 Reviews

TransIT®-293 Transfection Reagent

Mirus Bio LLC

A high efficiency, low toxicity, DNA transfection reagent optimized for 293 cellsTransIT®-293 Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HEK 293 and cell types of associated lineage. TransIT-293 provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…

5.0/5.0
|1 Review


MycoAlert™ PLUS Mycoplasma Detection Kit

Lonza Group Ltd

The MycoAlert™ PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlert™ PLUS Substrate, c…

4.6/5.0
|4 Reviews






NEBuilder® HiFi DNA Assembly Cloning Kit

New England Biolabs Inc.

NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, a…

5.0/5.0
|7 Reviews

Quick Ligation™ Kit

New England Biolabs Inc.

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C ) Fast - 5 minutes for cohesive or blunt ends. Convenient - ligation performed at room temperature. Flexible - suitable for all common ligation reactions.

5.0/5.0
|3 Reviews


pGLO™ Bacterial Transformation Kit

Bio-Rad

Genetic engineering is the process of manipulating the genetic material of an organism — often to include the DNA from a foreign organism. Using the classic pGLO Bacterial Transformation Kit, students transform bacteria by introducing a gene from the bioluminescent jellyfish Aequorea victoria. The same procedure has been used to create "designer proteins" which have led to the explosion of new health treatments, agricultural a…

4.7/5.0
|2 Reviews

mMESSAGE mMACHINE™ T7 Transcription Kit

Thermo Fisher Scientific

mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its s…

4.7/5.0
|1 Review

SHERLOCK™

Sherlock Biosciences

SHERLOCK is an evolution of CRISPR technology, which others use to make precise edits in genetic code. SHERLOCK can detect the unique genetic fingerprints of virtually any DNA or RNA sequence in any organism or pathogen. Developed by our founders and licensed exclusively from the Broad Institute, SHERLOCK is a method for single molecule detection of nucleic acid targets and stands for Specific High Sensitivity Enzymatic Report…

5.0/5.0
|1 Review

CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 50 rxns

EpiCypher

Recombinantly produced in E. coli, CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUTANA™ pAG-MNase contains a C-terminal 6xHis epitope tag. CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows Formulation: CUTANA™ pAG-MNase is provided as a 20…

4.7/5.0
|1 Review

Gibson Assembly® Master Mix

New England Biolabs Inc.

Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Assembly and transformation in just under two hours Flexible sequence design (scar-less cloning) No PCR clean-up step required High transformation efficiencies for inserts up to 20 kb Easily adapted for multiple DNA manipulations, including site-directed mutagenesis

5.0/5.0
|1 Review