Next-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl…
The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents…
The MiSeqDx instrument is the first and only FDA-cleared in vitro diagnostic (IVD) next-generation sequencing (NGS) system. Designed specifically for the clinical laboratory environment, the MiSeqDx instrument offers a small, approximately 4 square feet (0.37 square meters) footprint, an easy-to-follow workflow, and data output tailored to the needs of clinical labs. In addition, the integrated software enables sample trackin…
The NGS STARlet is a compact, flexible and future-proof workstation for library preparation for all common sequencing platforms. It is excellently suited for any throughput from 8 to 48 samples and reduces time consuming manual pipetting steps and sample-to-sample variability. The open workstation concept allows automation of any library preparation chemistry. Already included are validated methods for the NEBNext® Ultra DNA l…
Our latest generation Ovation RNA-Seq System V2 provides a fast and simple method for preparing amplified cDNA from total RNA for RNA-Seq applications, now with enhanced transcript coverage and uniform distribution of sequencing reads. Amplification is initiated at the 3' end as well as randomly throughout the whole transcriptome in the sample. This feature makes the Ovation RNA-Seq System V2 ideal for amplification prior to…
SURVEYOR Mutation Detection Kits provide a simple, accurate and cost-effective means to scan DNA fragments for mutations with unmatched sensitivity and specificity. The SURVEYOR Mutation Detection Kit for Standard Gel Electrophoresis has been designed to cleave unlabeled DNA fragments at mismatched sites for subsequent analysis by agarose gel electrophoresis or polyacrylamide gel electrophoresis (PAGE). DNA 200 to 4,000 bp lo…
SUPREMERUN is optimised for Sanger sequencing of your individual research samples in tubes or 96 well plates.
LIGHTRUN is our most convenient Sanger sequencing solution for your premixed samples.
Highlights: Fastest exome sequencing, starting from one sample Applicable for genomic DNA from various sources (tissue, cells, blood and FFPE sample) Special protocol for low-input samples (≥ 10ng) Available under diagnostics standard (ISO17025) INVIEW™ stands for streamlined high-quality next generation sequencing (NGS) solutions for specific applications. Exome enrichment with the latest Agilent SureSele…
Introducing the next-generation sequencing system for the molecular diagnostic laboratory. The Ion PGM™ Dx Instrument System* will deliver the speed and simplicity of the Ion Torrent™ platform, combined with key workflow advantages designed specifically for regulated laboratory environments and in vitro diagnostic applications. Used in conjunction with Ion PGM™ Dx library kits, Ion One Touch™ Dx template kits,…
