High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The 1290 Infinity II Multisampler is a multipurpose autosampler that handles vials and microplates. Injecting at pressures up to 1300 bar, the Multisampler is a compact module with a capacity of up to 6,144 samples, all within the footprint of an Agilent LC stack.
DURAN® HPLC Bottle with 4 port GL45 Cap.Safe transfer of HPLC liquids and also used for sterile transfer of liquid media. Complete system including DURAN® Pressure Plus Bottle plus 4 port GL45 PP screw cap. DURAN Pressure Plus bottles are designed and manufactured with a modified geometry for pressure/vacuum resistance from -1 to +1.5 bar in accordance with ISO 4796-1. With pressure load the following apply; thermal shock res…
Ascentis Express RP-Amide HPLC columns are the most recent product additions to the Supelco HPLC product line. Combining an embedded polar group (EPG) stationary phase with the Fused-Core particles, Ascentis Express RP-Amide provides a host of useful benefits to the HPLC chromatographer. Ascentis Express RP-Amide is a blend of modern phase technology and innovative particle technology. The Fused-Core particle provides…
World-class Small Multi Detection GPC System. A refractive index detector is generally used for GPC analysis of hydrophobic polymers. When trace-level additives that have UV absorption are contained in a polymer, both a UV detector and a photodiode array detector, which offers high-sensitivity detection, are used complementarily.By using the Prominence-i (PDA model), LabSolutions workstation, and LabSolutions GPC software (op…
C18 HPLC column for High Speed and High Resolution Applications. Ascentis® Express C18 is the first choice for starting a new method. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles at lower system pressure. With identical conditions, the Ascentis Express C18 column performs more than 4X faster tha…
Classic reversed-phases for all general-purpose applications. Features: Excellent peak shape and efficiency compared to competitive columns Classic reversed-phase retention and selectivity Excellent, no-bleed character for LC-MS applications C18 is generally more retentive than the C8 Column Details: Chemistry: C18 Particle Size: 5 µm Pore Size: 180 Å Length: 20, 30, 50, 100, 125, 150, 250 mm ID: 2.1, 3.0, 4.…
The LaChrom Elite Autosampler represents a new standard of performance in HPLC. Whether you need high sample capacity, extreme precision, ultra-low carry over, or rapid cycle times between injections, the LaChrom Elite models excel.
The use of an ultra pure, ultra inert silica has many recognised benefits, including improved reproducibility, lifetime and chromatographic performance (particularly with basic molecules). However, since the ultra inert silica surface effectively no longer contributes to the separation, C18 columns manufactured with high purity silicas show near identical selectivity. It is therefore highly likely that a problem separation on…
The LC-4000 series HPLC pumps include expansion bays that allow a simple pump module to be transformed into a powerful solvent delivery system.Accessory modules can be installed in the LC-4000 series pump for use with virtually any solvent delivery requirement. Features Upgrade and Expansion Eliminating pulsation Pump configurations Solvent Mixing Solvent Degassing Preparative and Semi-preparative pumps
The LC-4000 Series autosamplers provide a range of options to suit any sample injection requirements, from the AS-4050 entry-level model, to the AS-4250 UHPLC model or the AS-4058 preparative scale autosampler. All LC-4000 series autosamplers share the same simple maintenance features, with consumable parts such as injection ports, rotor seals, and loops accessible from the front panel for easy replacement.
The LC-4000 Series column ovens have been designed to provide excellent utility for a wide range of different user requirements.For simple requirements the stackable CO-4061 can be used with column lengths up to 200mm (or up to 300mm with an optional extension). For RHPLC and UHPLC the CO-4062 has capacity for up to six 150 mm length columns, with optional one or two internally mounted automatic switching valves for 2 or 6 co…
If you want to solve complex separations, analyze complex samples, or simplify complex workflows, Agilent InfinityLab 2D-LC Solutions help you separate co-eluting compounds and achieve highest peak capacity. Increase resolution where you need it with heart-cutting and high-resolution sampling or comprehensive 2D-LC for complex mixtures. Use 2D-LC for making buffer separations compatible to MS detection.
InfinityLab Quick Connect fittings are the only truly "finger-tight," reusable fitting for UHPLC, stable to 1300 bar. There are no tools needed… just use your fingers to close the lever for a perfect connection every time.
Polymer-based columns for aqueous Size Exclusion Chromatography (GFC) suitable for light scattering detector.
Polymer-based columns for aqueous Size Exclusion Chromatography (GFC).
Polymer-based columns for Hydrophilic Interaction Chromatography.
In order to more easily isolate and identify proteins in human serum, the Agilent Human 14 Multiple Affinity Removal Column is designed to chromatographically remove fourteen interfering high-abundant proteins from human plasma samples.
Sugar series columns using dual mode of Ligand Exchange and Size Exclusion Chromatography for specific separation of sugars. Separates mono and di-saccharides by combination of ligand exchange and size exclusion mode Counter ion: Ca2+ (divalent metal ions attached to the packing material separate saccharides from sugar alcohol as these columns retain sugar alcohol strongly.) Only water is required for the analysis (c…
Process and interpret all your analytical data in one common interface
Simplifies Clean-Up and Degassing of Mobile Phase Solvents and Other Solutions Versatile design fits most HPLC bottles, flasks, and containers, and eliminates the added steps of washing flasks and transferring mobile phase solvent from flask to reservoir. Draws directly from HPLC solvent bottle. Less likely to spill aggressive solvents than glass funnels or disposable cups. Durable plastic construction is less likely…
The 1260 Infinity II Prime LC is a versatile HPLC offering the highest functionality and operational convenience for analytical HPLC and entry-level quaternary UHPLC at pressures up to 800 bar and for flow rates up to 5 mL/min. This versatile HPLC system is a perfect front end for your MS system.
