New England Biolabs Inc. Products & Reviews

Quickly and easily purify high quality DNA from PCR and other enzymatic reactions.

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. Working with uracil-…

Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Assembly and transformation in just under two hours Flexible sequence design (scar-less cloning) No PCR clean-up step required High transformation efficiencies for inserts up to 20 kb Easily adapted for multiple DNA manipulations, including site-directed mutagenesis

The unique workflow of the NEBNext Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.  

Quickly and easily purify DNA from agarose gels with high yields.

For the extraction of HMW DNA from a variety of tissues, bacteria and other samples (yeast, insect, amphibian)

Gibson Assembly® allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.

NEBNext® Enzymatic Methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.

Restriction Enzyme 

the BglII restriction endonuclease is derived from an  E. coli  strain that carries the BglII gene from  Bacillus globigii  (ATCC 49760).

BL21 Competent E. coli is a widely used non-T7 expression E. coli strain and is suitable for transformation and protein expression. This strain does not express the T7 RNA Polymerase. Ideal for Plac, Ptac, Ptrc ParaBAD expression vectors Protease deficient No dry ice surcharge on competent cell shipments Outgrowth medium included Free of animal products

DNA specific exonuclease Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5' to 3' direction Highly processive degradation of double-stranded DNA from the 5' end Preferred substrate is 5'-phosphorylated double-stranded DNA although non-phosphorylated substrates are degraded at a greatly reduced rate Lambda Exonuclease is ideal for: Conversion of linear double-stranded DN…

Rapid, sensitive, and precise probe-based qPCR detection and quantitation of target RNA targets.

T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA.

BbsI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10119525.  NEB is excited to announce that all reaction buffers are now BSA-free. NEB began switching their BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases (kb). The 3.0 kb fragment has increased intensity to serve as a reference band. The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) f…

phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5´ proofreading exonuclease activity. Source: An E. coli strain that carries the phi29 DNA Polymerase gene from bacteriophage phi29 Applications: •Replication requiring a high degree of strand displacement and/…

The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® contains enzymes and buffers that are ideally suited for cDNA library preparation for next-generation sequencing. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mi…

Western blotting is a widely used technique to detect levels of protein expression in cell or tissue extract. This technique measures protein levels in a biological sample through antibody binding to a specific protein of interest.   Features: Detects Small Amounts of Protein – delivers a sensitive and robust signal for standard protein detection Convenient, Cost-effective 500 ml Sizing – means you can p…

Streptavidin Magnetic Beads are 1 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids.

The EnGen sgRNA Synthesis Kit,  S. pyogenes  provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.

Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies.  Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.  Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart™ Buffer (over 215 enzymes are available in the same b…

For Research Use Only. Not for use in diagnostic procedures.

Gel Loading Dye, Purple (6X) is the premier gel loading dye from NEB for sharp, tight bands.

The NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina offers an amplification-free workflow for DNA-seq based on the streamlined and reliable Ultra II FS workflow. Starting with as little as 50 ng of DNA, get the high-quality, high-yield libraries that you need without PCR bias.