Products & Reviews

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences

PvuII has a High Fidelity version PvuII-HF® (NEB #R3151) High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.  HF enzymes also exhibit dramatically reduced star activity.  HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without de…

The Quick Dephosphorylation Kit contains Quick CIP, a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.

NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, a…

The New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for autom…

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C ) Fast - 5 minutes for cohesive or blunt ends. Convenient - ligation performed at room temperature. Flexible - suitable for all common ligation reactions.

An optimized blend of Taq and Deep Vent® DNA Polymerases, LongAmp® Taq DNA Polymerase enables amplification of up to 30 kb PCR products with a fidelity higher than Taq DNA Polymerase alone. LongAmp Taq is also available with Crimson tracking dye (NEB #M0326) in the reaction buffer.

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. Time-Saver™  qualified for digestion in 5-15 minutes High Fidelity (HF®) version available (NEB #R3189) supplied with CutSm…

The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.