Gene Expression and Molecular Cloning Products & Reviews

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Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Visium Spatial Gene Expression

10x Genomics

Visium Spatial Gene Expression is a next-generation molecular profiling solution for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context in FFPE or fresh-frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research.

4.9/5.0
|4 Reviews


LightCycler ® 96 instrument

Roche

The LightCycler® 96 Instrument is a real-time PCR instrument for rapid cycling up to 96 samples. Applications include absolute and relative quantification, melting curve analysis, and endpoint genotyping. Precise LightCycler® 96 Instrument thermal homogeneity and cycling speed produce accurate and reproducible results in a very short time. The LightCycler® 96 Instrument’s optical detection system flexibly detects sequenc…

4.9/5.0
|2 Reviews


High Capacity RNA-to-cDNA Master Mix

Thermo Fisher Scientific

The High Capacity RNA-to-cDNA Master Mix optimizes cDNA synthesis for high-throughput, real-time gene expression analysis. • Single tube, complete first-strand cDNA synthesis master mix reduces experimental variability and streamlines workflow • 5X concentration provides robust results even with dilute RNA samples and rare or abundant transcripts • Liquid format at –10 and –20°C eliminates freeze/thaw cycles and maximizes chem…

5.0/5.0
|1 Review

High Sensitivity ß-Galactosidase Assay Kits

Agilent Technologies

The High Sensitivity ß-Galactosidase Assay Kit provides an easy, rapid, and sensitive method for determining the ß-galactosidase activity in the lysates of cells transfected with a ß-galactosidase expression construct. Rapid, simple and sensitive >= 10-fold increase in sensitivity over ONPG-based kits Reduces amount of cell extract needed for assay Useful for cells that are difficult to transfect and have low ß-gal expression…

5.0/5.0
|1 Review

GFP Variant Vectors

Takara Bio

These bacterial expression vectors carry the gene for either GFP (wild type Aequorea victoria green fluorescent protein) or GFPuv (a GFP variant optimized for maximal fluorescence when excited by UV light [360–400 nm]) and are driven by the lac promoter. These vectors can also be used as a source of the indicated fluorescent protein coding sequences. The fluorescent protein gene can be amplified by PCR or readily excised using…

5.0/5.0
|1 Review

INTERFERin™-HTS

Polyplus Transfection

INTERFERin™-HTS is a new generation siRNA transfection reagent especially developed for high throughput screening (HTS) applications providing great silencing efficiency, excellent reproducibility and high cell viability with very low amounts of reagent. INTERFERin™-HTS is cost-effective, easy to handle, compatible with serum and antibiotics, and comes with reverse and forward protocols for 96- and 384-well plates. Great s…

5.0/5.0
|1 Review



SEQuoia Express Stranded RNA Library Prep Kit

Bio-Rad

SEQuoia Express Stranded RNA Library Prep Kit enables customers to overcome the challenge of a laborious library prep workflow by providing a cost-effective 3-hour, 3-tube, automation-friendly solution for constructing robust libraries that efficiently capture long RNA transcripts (>200bp).

4.0/5.0
|1 Review
  • Best New Life Sciences Product of the Year
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P-1000 Next Generation Micropipette Puller

Sutter Instrument Co.

Developed through years of experience with the Flaming/Brown style micropipette pullers and infused with leading edge technology, Sutter is proud to introduce the P-1000 micropipette puller. The most obvious new feature is the color touchscreen display that provides an intuitive and full-featured interface. Key Features: Color touch-screen interface Safe heat mode to protect and extend filament life Line repeat mode sim…

4.5/5.0
|5 Reviews
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P-2000 Laser Based Micropipette Puller

Sutter Instrument Co.

A significant advance in the technology of fabrication of micropipettes, optical fiber probes, and nanospray tips, is offered with the P-2000 micropipette puller. The P-2000 integrates a CO2 laser-based heat source with the technology derived from our extensive experience with conventional pullers. This system offers capabilities unmatched by other pullers. Key Features: Capable of pulling quartz, borosilicate and alumino…

3.7/5.0
|1 Review
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P-97 Flaming/Brown Micropipette Puller

Sutter Instrument Co.

The Model P-97 micropipette puller is the latest generation of the Flaming/Brown type puller for fabrication of micropipettes, patch pipettes and microinjection needles. While retaining many of the features on the Model P-87, the P-97 offers improvements in mechanical, electronic and software design. The result is better control of the pulling process and a higher degree of reproducibility. Features: Environmental chamber…

4.3/5.0
|3 Reviews
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FuGENE ® HD Transfection Reagent

Promega Corp.

FuGENE® HD Transfection Reagent is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in che…

4.3/5.0
|6 Reviews
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Dual-Luciferase ® Reporter Assay System

Promega Corp.

The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at l…

4.4/5.0
|9 Reviews
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Steady-Glo ® Luciferase Assay System

Promega Corp.

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample…

4.5/5.0
|5 Reviews
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Dual-Glo® Luciferase Assay System

Promega Corp.

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize s…

4.2/5.0
|5 Reviews
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Nucleofector® II Device

Lonza Group Ltd

The basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format. What benefits are important for your work? Efficient transfection in single cuvette format? Up to 90% efficiency with plasmid DNA…

4.2/5.0
|6 Reviews


Medicago Gene Expression Microarray, 4x44k

Agilent Technologies

Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunoprecipitation (ChIP) pull-down coupled with powerful DNA microarray location analysis. Beyond embryonic and adult development gene regulation, Agilent™s ChIP-on-chip model systems effectively elucidates the vital biological processes.  Probe number depends on species  4x44k and…

4.0/5.0
|1 Review

Rabbit Gene Expression Microarrays

Agilent Technologies

The Agilent Rabbit Microarray delivers accurate, consistent and efficient gene expression profiling of rabbit samples. The transcriptome-wide microarrays enable scientists to study gene expression profiling of Oryctolagus cuniculus samples at the global level and enhance the understanding of gene function.

4.3/5.0
|1 Review