Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Roche FuGENE HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. Roche FuGENE HD Transfection Reagent is designed to transfect a wide range of eukaryotic cells, including insect cells and many tumor cell lines not transfected well by other reagents (e.g., MCF7, Hep G2, PC-3, HeLa, MA-10, Hep G2, SH-SY5Y, A7r5, STO, SCC-61, SQ20B, T98G, STSAR-90, A375, A549, and others). In addi…
The Nucleofector technology is a highly efficient non-viral method for transfection. It is based on two components: the Nucleofector Device that delivers unique electrical parameters, and Nucleofector Kits that contain cell-type-specific Nucleofector Solutions. The electrical parameters of the Nucleofector are different from any other commercially available electroporation device. Each electrical setting is displayed as a dist…
The New Standard for Desktop-Based Bioinformatics Tools CLC DNA Workbench provides a software environment which enables users to perform advanced DNA sequence analyses such as assembly of DNA sequence data, graphically and algorithmically advanced primer design, while offering user-friendly molecular cloning tools. CLC DNA Workbench is a bioinformatics software package containing a range of specialized DNA analyses and bioinfo…
Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. Features: High yields of capped RNA—up to 39 µg of RNA per reaction Extra convenience—cap analog-nucleotide mix provided Simplified rea…
Introducing the versatile reagent with the power to transfect your most difficult cells.
Contains one vial of Lipofectamine® 3000 Reagent (1.5 mL) and one vial of P3000™ Enhancer Reagent.
FuGENE® HD Transfection Reagent is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in che…
Ready-to-use, easy interpretation of results, Highly-sensitive, no need for internal control
Universal, clinically applicable enhancer solution for lentivirus based transductions.
Gene Pulser®/Micropulser™ electroporation cuvettes are high-quality electroporation cuvettes which enable reproducible and consistent gene delivery to valuable transfection samples.
SEQuoia Express Stranded RNA Library Prep Kit enables customers to overcome the challenge of a laborious library prep workflow by providing a cost-effective 3-hour, 3-tube, automation-friendly solution for constructing robust libraries that efficiently capture long RNA transcripts (>200bp).
The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at l…
High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample…
Versatile — works with a broad range of cell lines Reproducible — produces consistent results, lot-to-lot, plate-to-plate, and well-to-well Efficient — performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects Fast — reverse-transfects cells as they are plated, saving a day
Roche FuGENE 6 Transfection Reagent is a non-liposomal multicomponent reagent well-suited for experiments involving cellular analysis. Due to the simple methodology of FuGENE 6 Transfection Reagent, minimal optimization, low cytotoxicity, and ability to provide high transfection efficiency even in the presence of serum, it is one of the standard transfection reagents in many different laboratories around the world. With a ment…
High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix and others. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template quantities. The new High Capacity RNA-to-cDNA formulation reduces reverse transcription time by…
TaqMan® MicroRNA Arrays provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan MicroRNA Assays in parallel. Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the TaqMan MicroRNA Array. When sensitivity is of th…
The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize s…
Roche NimbleGen provides a unique combination of high-density arrays, long-oligo probes, and flexible design capability that together provide superior results for advanced gene expression analysis. NimbleGen microarrays enable accurate, sensitive, and specific interrogation of genome-wide expression for any sequenced and annotated genome. Researchers also can tile through a genome at any desired spacing to discover genome-wide…
Key Features of the cloning technology Clone any insert, at any site within any vector Restriction enzyme, phosphatase and ligase free system Powerful technology to join multiple fragments at once Rapid one reaction for 5 minutes at room temperature, then fusion on ice for 10 minutes Introduce or correct mutations at will in cDNAs Amenable to high throughput cloning applications
Exiqon miRCURY LNA™ First-strand cDNA Kit First-strand cDNA synthesis reagents for high performance microRNA real-time PCR using the miRCURY LNA™ microRNA PCR system. Features of the Exiqon miRCURY LNA™ First-strand cDNA Kit: • Optimized for fast, easy and reliable first-strand cDNA synthesis using our miRCURY LNA™ microRNA PCR system • Includes a thermostable and highly reliable reverse transcriptase The miRCURY LNA™ Fir…
