Gene Expression and Molecular Cloning Products & Reviews

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Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Fermentas* TurboFect* Protein Transfection Reagent

Thermo Fisher Scientific

In combination with the included Enhancer Solution, the reagent efficiently delivers functionally active proteins, antibodies and peptides into the cytoplasm of cells. Proteins of different sizes and net charge (different pI) can be delivered into various cell types, including primary, adherent and suspension cells (see Fig. 1, 2 and list of cells below). The reagent can be used in the presence or absence of serum. TurboFect P…

4.3/5.0
|1 Review

Yeastmaker Yeast Transformation System 2

Clontech Laboratories, Inc.

The Yeastmaker Yeast Transformation System 2 provides a high-efficiency polyethylene glycol (PEG)/LiAc-based method for preparing and transforming competent yeast cells. The Yeast Transformation System 2 protocol provides a higher and more reliable transformation efficiency than many other commonly used methods as a result of specially formulated "YPD Plus" recovery medium, which results in more surviving transformed colonies…

4.0/5.0
|1 Review

StrataClone PCR Cloning Kits

Agilent Technologies

The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. 

3.8/5.0
|2 Reviews



DNA Ligation Kit Mighty Mix

Takara Bio

The DNA Ligation Kit Mighty Mix is a single solution, ready-to-use 2X ligation mix that enables high efficiency ligations, particularly for blunt-ended ligations. This DNA ligation kit can also be used for ligation into TA-vectors. The basic ligation reaction can be quickly performed, either for 5 minutes at 25°C or for 30 minutes at 16°C, with equal results for both circular and linear DNA ligations. The 2X Ligation Mix is c…

4.3/5.0
|1 Review

FuGENE® HD Transfection Reagent

Roche FuGENE HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. Roche FuGENE HD Transfection Reagent is designed to transfect a wide range of eukaryotic cells, including insect cells and many tumor cell lines not transfected well by other reagents (e.g., MCF7, Hep G2, PC-3, HeLa, MA-10, Hep G2, SH-SY5Y, A7r5, STO, SCC-61, SQ20B, T98G, STSAR-90, A375, A549, and others). In addi…

4.2/5.0
|6 Reviews

The Nucleofector Device

Lonza Group Ltd

The Nucleofector technology is a highly efficient non-viral method for transfection. It is based on two components: the Nucleofector Device that delivers unique electrical parameters, and Nucleofector Kits that contain cell-type-specific Nucleofector Solutions. The electrical parameters of the Nucleofector are different from any other commercially available electroporation device. Each electrical setting is displayed as a dist…

3.7/5.0
|12 Reviews

CLC DNA Workbench Software

CLC bio

The New Standard for Desktop-Based Bioinformatics Tools CLC DNA Workbench provides a software environment which enables users to perform advanced DNA sequence analyses such as assembly of DNA sequence data, graphically and algorithmically advanced primer design, while offering user-friendly molecular cloning tools. CLC DNA Workbench is a bioinformatics software package containing a range of specialized DNA analyses and bioinfo…

4.3/5.0
|2 Reviews

mMESSAGE mMACHINE® T3 Transcription Kit

Thermo Fisher Scientific

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. Features: High yields of capped RNA—up to 39 µg of RNA per reaction  Extra convenience—cap analog-nucleotide mix provided  Simplified rea…

4.3/5.0
|1 Review









siPORT™ NeoFX™ Transfection Agent

Ambion Inc

Versatile — works with a broad range of cell lines Reproducible — produces consistent results, lot-to-lot, plate-to-plate, and well-to-well Efficient — performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects Fast — reverse-transfects cells as they are plated, saving a day

3.8/5.0
|4 Reviews

FuGENE 6 Transfection Reagent

Roche FuGENE 6 Transfection Reagent is a non-liposomal multicomponent reagent well-suited for experiments involving cellular analysis. Due to the simple methodology of FuGENE 6 Transfection Reagent, minimal optimization, low cytotoxicity, and ability to provide high transfection efficiency even in the presence of serum, it is one of the standard transfection reagents in many different laboratories around the world. With a ment…

3.8/5.0
|16 Reviews

High Capacity RNA-to-cDNA Kit

Thermo Fisher Scientific

High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix and others. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template quantities. The new High Capacity RNA-to-cDNA formulation reduces reverse transcription time by…

4.3/5.0
|1 Review

TaqMan® MicroRNA Arrays

Thermo Fisher Scientific

TaqMan®  MicroRNA Arrays provide all the advantages of TaqMan®  MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan MicroRNA Assays in parallel. Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the TaqMan MicroRNA Array. When sensitivity is of th…

4.3/5.0
|3 Reviews

NimbleGen Gene Expression Microarray

Roche

Roche NimbleGen provides a unique combination of high-density arrays, long-oligo probes, and flexible design capability that together provide superior results for advanced gene expression analysis. NimbleGen microarrays enable accurate, sensitive, and specific interrogation of genome-wide expression for any sequenced and annotated genome. Researchers also can tile through a genome at any desired spacing to discover genome-wide…

4.0/5.0
|1 Review

Cold Fusion Cloning Kit

System Biosciences (SBI)

Key Features of the cloning technology Clone any insert, at any site within any vector Restriction enzyme, phosphatase and ligase free system Powerful technology to join multiple fragments at once Rapid one reaction for 5 minutes at room temperature, then fusion on ice for 10 minutes Introduce or correct mutations at will in cDNAs Amenable to high throughput cloning applications

4.3/5.0
|1 Review

miRCURY LNA™ First-strand cDNA Kit

Exiqon

Exiqon miRCURY LNA™ First-strand cDNA Kit First-strand cDNA synthesis reagents for high performance microRNA real-time PCR using the miRCURY LNA™ microRNA PCR system. Features of the Exiqon miRCURY LNA™ First-strand cDNA Kit: • Optimized for fast, easy and reliable first-strand cDNA synthesis using our miRCURY LNA™ microRNA PCR system • Includes a thermostable and highly reliable reverse transcriptase The miRCURY LNA™ Fir…

4.0/5.0
|1 Review