Cloning Kits Products & Reviews

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Cloning Kits

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LigaFast™ Rapid DNA Ligation System

Promega Corp.

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).   Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…

4.7/5.0
|2 Reviews
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StrataClone Blunt PCR Cloning Kit

Agilent Technologies

The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. These kits are available for both blunt-end and UA cloning. 

5.0/5.0
|1 Review


NEBuilder® HiFi DNA Assembly Cloning Kit

New England Biolabs Inc.

NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, a…

5.0/5.0
|7 Reviews

Quick Ligation™ Kit

New England Biolabs Inc.

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C ) Fast - 5 minutes for cohesive or blunt ends. Convenient - ligation performed at room temperature. Flexible - suitable for all common ligation reactions.

5.0/5.0
|3 Reviews

mMESSAGE mMACHINE™ T7 Transcription Kit

Thermo Fisher Scientific

mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its s…

4.7/5.0
|1 Review

Gibson Assembly® Master Mix

New England Biolabs Inc.

Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Assembly and transformation in just under two hours Flexible sequence design (scar-less cloning) No PCR clean-up step required High transformation efficiencies for inserts up to 20 kb Easily adapted for multiple DNA manipulations, including site-directed mutagenesis

5.0/5.0
|1 Review




SEQuoia Express Stranded RNA Library Prep Kit

Bio-Rad

SEQuoia Express Stranded RNA Library Prep Kit enables customers to overcome the challenge of a laborious library prep workflow by providing a cost-effective 3-hour, 3-tube, automation-friendly solution for constructing robust libraries that efficiently capture long RNA transcripts (>200bp).

4.0/5.0
|1 Review
  • Best New Life Sciences Product of the Year
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StrataClone PCR Cloning Kits

Agilent Technologies

The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. 

3.8/5.0
|2 Reviews



DNA Ligation Kit Mighty Mix

Takara Bio

The DNA Ligation Kit Mighty Mix is a single solution, ready-to-use 2X ligation mix that enables high efficiency ligations, particularly for blunt-ended ligations. This DNA ligation kit can also be used for ligation into TA-vectors. The basic ligation reaction can be quickly performed, either for 5 minutes at 25°C or for 30 minutes at 16°C, with equal results for both circular and linear DNA ligations. The 2X Ligation Mix is c…

4.3/5.0
|1 Review

mMESSAGE mMACHINE® T3 Transcription Kit

Thermo Fisher Scientific

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. Features: High yields of capped RNA—up to 39 µg of RNA per reaction  Extra convenience—cap analog-nucleotide mix provided  Simplified rea…

4.3/5.0
|1 Review


Cold Fusion Cloning Kit

System Biosciences (SBI)

Key Features of the cloning technology Clone any insert, at any site within any vector Restriction enzyme, phosphatase and ligase free system Powerful technology to join multiple fragments at once Rapid one reaction for 5 minutes at room temperature, then fusion on ice for 10 minutes Introduce or correct mutations at will in cDNAs Amenable to high throughput cloning applications

4.3/5.0
|1 Review






pCMV-Script Vectors

Agilent Technologies

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.

0.0/5.0
|0 Reviews