Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Efficient siRNA or microRNA transfection. To attain efficient and reliable siRNA or microRNA transfection, we offer DharmaFECT transfection reagents. Thermo Scientific DharmaFECT 1 is our most all-purpose transfection reagent formulation; demonstrating efficient, low-toxicity delivery to over 35 cell types. DharmaFECT 1 achieves effective transfection of siRNA and microRNA reagents at low concentrations and with low cytotoxic…
SmartRNAplex miRNA Assays are your benchtop solution for miRNA profiling, using a familiar protocol, and simple acquisition software. SmartRNAplex microRNA Assays use proprietary Firefly™ technology, which exploits uniquely encoded hydrogel particles developed by innovators at Firefly BioWorks. The Firefly™ hydrogel particles are hydrophilic, bio-inert, flexible and porous, allowing target molecules to diffuse and bind in a…
The TnT® Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix. The TnT® Quick Coupled Transcription/Translation System is ava…
The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. Kazusa DNA Research Institute (KDRI) is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. KDRI is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Uni…
High yield transient transfection and baculovirus titers in insect cells. Insect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression.TransIT®-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression i…
Research and Clinical Grade RetroNectin Reagent for Retroviral or Lentiviral Transduction. RetroNectin Reagent for retroviral or lentiviral transduction significantly increases transduction efficiency and results in enhanced gene transfer, especially in suspension and hematopoietic stem cells. RetroNectin Reagent is a chimeric peptide composed of E. coli-derived recombinant human fibronectin fragment that contains three funct…
Mouse ROSA26 safe harbor gene knock-in kit and clones. ROSA26 (also known as ROSAβgeo26 locus) in mice genome is first found in chromosome 6 in one particular strain of mice-named ROSAβgeo26-expressed β galactosidase from a randomly inserted transgene at high levels uniformly in nearly all tissues examined. This locus expresses one coding transcript and two noncoding transcripts, and only the non-coding transcripts are disrup…
GeneCopoeia offers complete custom services for CRISPR-Cas9-based targeted genomic modification, including CRISPR design and construction, donor design and construction, functional validations, stable cell line establishment, and transgenic mouse generation. Service Details: Validation Services Donor clone services Stable cell line services Transgenic mouse services Advantages: Fast delivery of CRISPR-Cas9 plasmids…
SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first strand synthesis, without adaptor ligation. The presence of these known sequences is crucial for a number of downstream applications including amplification, RACE, and library construction. While a wide variety of technologies can be employed to take advantag…
With over 20 million pre-made clones representing nearly every known gene for most common organisms, Source BioScience are your perfect partner for quick and easy solutions for gene and protein expression research. Source BioScience customers can choose from individual pre-made clones or entire clone libraries from many of the most well-known and cited clone consortia and research groups for mammalian and non-mammalian specie…
Source BioScience are a trusted provider of outsourced services for gene expression projects. Source BioScience work with three major microarray platforms (Affymetrix, Agilent and Illumina) and optimise the most suitable array technology for each project.
Takara's DNA Ligation Kit for Long Fragments is a powerful tool for the cloning of long DNA fragments spanning 2 kb to over 10 kb in length. The kit contains an optimized ligase/buffer system that enables ligation of long fragments without the need for extensive techniques or special expertise. It is especially well suited for BAC library construction. Features: Superior ligation efficiency, especially for long DNA frag…
Protein Analysis Software - the Next GenerationCLC Protein Workbench creates a software environment enabling users to make a large number of advanced protein sequence analyses, combined with smooth data management, and excellent graphical viewing and output options. Protein Workbench sets new standards for bioinformatics software with its overall graphical display, usability driven and user-friendly interface, while featuring…
Free Bioinformatics Software Not a demo. Not scaled down. No spyware. No need to register. Yet still a wide variety of features available - and - free! CLC Free Workbench creates a software environment enabling users to make a large number of bioinformatics analyses, combined with smooth data management, and excellent graphical viewing and output options. Some of the many bioinformatics analyses: • Interactive restriction site…
Contains one vial of Lipofectamine® 3000 Reagent (0.1 mL) and one vial of P3000™ Enhancer Reagent.
Contains 5x vial of Lipofectamine® 3000 Reagent (1.5 mL) and 5x vial of P3000™ Enhancer Reagent.
Contains one vial of Lipofectamine® 3000 Reagent (15 mL) and one vial of P3000™ Enhancer Reagent.
ScreenFect™ A is a cationic lipid transfection reagent combining high transfection efficiency with low cell toxicity.
Enabling generation of genome-wide binding profiles from as few as 10,000 cells
For use with The LightSwitch™ Luciferase Assay System for analysis of promoter regulation
For use with The LightSwitch™ Luciferase Assay System to measure the impact of a 3´UTR on post-transcriptional regulation
Designed to identify the physical interactions of a miRNA with endogenous mRNA transcripts
Complete solution for convenient, accurate monitoring of protein/DNA interactions
An optimized and streamlined protocol for high-resolution genome-wide mapping of transcription factor binding sites in the human and mouse genomes
Providing the highest quality ChIP-enriched DNA for analysis by qPCR or Next Generation sequencing
