Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
FlyFectin ™ is a specifically designed and very efficient transfection reagent for insect cells. Innovative and unique, FlyFectin ™ is: • Highly efficient with excellent reproducibility• Multipurpose (various types of nucleic acids)• Ideal for recombinant protein production with Baculovirus expression system• Simple, Ready-to-use and Rapid• Compatible with and without serum-containing culture media• Non toxic• Economi…
B-Pak, is a straightforward and rapid procedure for determining the concentration of protein in solution. Thanks to an optimized Bradford reagent formulation, B-Pak gives precise determination of protein concentration even in the presence of detergent, allowing the use of this kit for transfection experiments. In addition, this kit can be used for all protein concentration measurement due to a superior linearity of response: f…
Issued from Magnetofection™ technology, ViroMag is a specific formulation designed to be used in association with all viral vectors and for many transduction applications. For the first time, scientists will be able to increase transduction efficiency, infect non permissive cells, concentrate virus onto cells or in culture medium or synchronize infection without modification of the viruses, just by associating ViroMag reagent…
The pDual and pDual GC expression vectors are designed for high-level expression of heterologous genes in both eukaryotic and prokaryotic systems.High-level protein expression in bacterial or mammalian systems Saves time by eliminating the need to subclone Express protein as a native or tagged fusion protein Epitope tagged for simple detection and affinity purification Clone seamlessly without compatible restriction sites Avai…
An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…
VeroFect™ is a powerful reagent dedicated to the transfection of Vero cells. This lipid -based transfection reagent is issued from the revolutionary Tee-Technology and achieves up to 50-55% transfection efficiency. The complexes formed by DNA and VeroFect™ reagent have an exceptional capacity in destabilizing cell membranes, allowing the delivery of important DNA amounts into cells. VeroFect™ was specifically designed to obtai…
OnTarget™ is a powerful, new platform for detection of rare tumor mutations in tissue or plasma. • Detect 100 or more mutations in a single assay, without sample splitting • Quantify mutations present at 0.01% allele frequency and above • Enrich mutations extracted from plasma or FFPE in about 1 hour
High efficiency broad spectrum DNA transfection of cells in 3D culture for biologically relevant dataIdeal for the transfection of cells in 3D cell culture, the new 3D Transfection System combines the technologies of Reinnervate's alvetex® 3D Cell Culture Plates and Mirus Bio's TransIT®-3D Transfection Reagent, enabling scientists to create models that more accurately mimic the tissue environment, gaining a much deeper insight…
High efficiency electroporation using any instrumentElectroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity. Ingenio Kits and Solution are compatible with multiple conventional electroporation instruments including Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser and Harvard-BTX® electroporators.Ingenio® Cuvettes Features:•High effi…
High efficiency electroporation using any instrumentElectroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity.Ingenio® Cell Droppers Features:•High efficiency electroporation of hard to transfect cell lines and primary cells•Compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®•Idea…
High, reproducible protein yield in suspension CHO and 293 cellsTransIT-PRO® Transfection Kit consists of a DNA transfection reagent and boost combination specifically developed for mammalian protein production in suspension 293 and CHO derived cells.Reach critical stages faster. Decrease time to produce usable protein by maximizing target protein yields through transient transfection. The TransIT-PRO® Transfection Kit uses an…
A broad spectrum, low toxicity DNA transfection reagent optimized for 96-well plate applicationsTransIT®-Express Transfection Reagent is specifically developed for high-throughput transfection of plasmid DNA into mammalian cells. TransIT-Express Reagent provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use and repro…
A broad spectrum, low toxicity, DNA transfection reagentTransIT®-LT1 Transfection Reagent is a broad spectrum reagent that provides high efficiency plasmid DNA delivery in many mammalian cell types including primary cells. TransIT-LT1 is a low toxicity, serum-compatible transfection reagent that eliminates the need for any culture medium change.TransIT-LT1 is suitable for both transient and stable transfection and can be used…
A high performance, animal-free, broad spectrum DNA transfection reagentTransIT®-2020 Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells. TransIT-2020 affords high levels of gene expression in typically hard-to-transfect cell types, including primary cells and is suitable for both transient and stable transfection.Tra…
A high efficiency DNA transfection reagent optimized for Jurkat and other hard-to-transfect cell typesTransIT®-Jurkat Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Jurkat cells and cell types of associated lineage.TransIT-Jurkat reagent also works well for additional cell lines of hematopoietic origin such as THP-1(human acute monocytic leukemia cell line) and R…
A high efficiency, low toxicity, DNA transfection reagent optimized for prostate cell typesTransIT®-Prostate Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Prostate cell types. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use and reproducibil…
A high efficiency, low toxicity, DNA transfection reagent optimized for various neural cell typesTransIT-Neural® Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in various neural cell types. TransIT-Neural Reagent provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simpli…
A high efficiency, low toxicity, DNA transfection reagent optimized for CHO cell typesTransIT®-CHO Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in CHO cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…
A high efficiency, low toxicity, DNA transfection reagent optimized for 3T3 cell typesTransIT®-3T3 Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in NIH 3T3 cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of…
A high efficiency, low toxicity, DNA transfection reagent optimized for COS cell typesTransIT®-COS Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in COS cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…
A high efficiency, low toxicity, DNA transfection reagent optimized for keratinocyte cellsTransIT®-Keratinocyte Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in keratinocyte cell types such as NIKS (Near-Diploid Immortalized Keratinocytes) and primary keratinocytes. TransIT-Keratinocyte Reagent provides all the attributes of the trusted TransIT series of transfecti…
A high efficiency, low toxicity, DNA transfection reagent optimized for HeLa cellsTransIT-HeLaMONSTER® Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HeLa cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity o…
Superior DNA transfection of breast cancer cell typesTransIT-BrCa Transfection Reagent is the first dedicated breast cancer cell line specific reagent available to researchers today. While it delivers efficiently to many breast cancer cell types, it also maintains low cellular toxicity resulting in reduced experimental biases. TransIT®-BrCa Transfection Reagent is specifically optimized to provide exceptional transfection effi…
A nontoxic, "Quick Recovery" In Vivo delivery solution that delivers DNA or siRNA to the livers of laboratory mice using methods including hydrodynamic tail vein injection.The TransIT-QR (Quick Recovery) Hydrodynamic Delivery Solution is designed specifically for the safe and efficient delivery of nucleic acids into laboratory mice using the hydrodynamic tail vein injection procedure. This formulation is optimized for efficien…
An "Enhanced Expression" In Vivo delivery solution that delivers DNA to the livers of laboratory mice using methods including hydrodynamic tail vein injection.The TransIT-EE (Enhanced Expression) Hydrodynamic Delivery Starter Kit is designed specifically for the delivery of DNA expression vectors into live laboratory mice using the hydrodynamic tail vein injection procedure. This formulation is designed to produce high-level g…
