PCR and Thermal Cycling Products & Reviews

UltraScript® cDNA Synthesis Kit is an easy-to-use 2-tube system specifically developed to generate cDNA for use in real-time PCR. The kit provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

PCRBIO 1-Step Go RT-PCR Kit is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube. The advanced buffer system, reverse transcriptase and hot start polymerase give highly specific and ultra-sensitive 1-step RT-PCR from any RNA template including mRNA, total RNA and viral RNA sequences.

qPCRBIO Probe 1-Step Virus Detect is designed for ultra-sensitive detection of viral RNA by 1-step RT-qPCR using sequence-specific probes.

Lyo-Ready Probe 1-Step Kit is a lyophilisable (freeze-dryable) RT-qPCR kit designed for ultra-sensitive detection of RNA sequences.

Air-Dryable Probe 1-Step Mix is a versatile all-in-one, air-dryable 4x RT-qPCR reagent mix for ultra-sensitive detection of RNA or DNA sequences.

RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara® Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.

Clara® HRM Mix delivers maximum specificity to your high resolution melt (HRM) curve analyses. Accurately detect genetic mutations, quickly identify genotypes based on SNPs, or calculate percent methylation of a target region with HRM analysis.

VeriFi® Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.

A comprehensive kit for efficient multiplex RT-qPCR in a single reaction tube. Ideal for multiplex (4-5) assays using dual-labelled hydrolysis probes, works with standard and fast cycling.

A comprehensive kit for performing dye-based RT-qPCR swiftly and reliably in a single tube. No carryover contamination and compatible with standard and fast cycling.

Reliable multiplex qPCR kit. Optimised for amplifying low copy DNA targets. Ideal for multiplexed reactions of 4 or 5 targets without compromising performance.

Ideal for quantification of any DNA template (gDNA, cDNA, viral DNA), amplifies low copy number samples with ease. Utilises our intercalating fluorescent dye, MicroGREEN, that binds to double stranded DNA, making it the perfect choice for qPCR & melt curve analysis.

Swift, cost-effective qPCR detection. Compatible with a wide range of qPCR instruments and mastermixes. Heightened sensitivity and outstanding reproducibility due to high fluorescence. A robust, thermally stable dye endures cycling and freeze-thaw cycles exceptionally well. 

The MegaMix Gold One-step RT-PCR kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit ensures a highly specific and sensitive amplification, removing background and primer dimer formation. MegaMix Gold boasts excellent accuracy and produces A-tailed products suitable for ligating into TA cloning vectors.

The Micronic Eco-Friendly Choice Rack RM is 99% made of renewed material, setting a new sustainable standard for sample storage racks.

The CpG WIZ® p16 Amplification Kit is used for determining the methylation status of the p16 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified such that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and unmethylated)…

The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unm…

The CpG WIZ® E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and…

The CpG WIZ® Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation -specific PCR (MSP). The kit contains primers targeted to the CpG island on the SNRPN gene. PCR parameters have been identified such that both primer sets in the kit amplify template under the same conditions. Normal control genomic DNA, which will generate a product with both primer sets, is also i…

CpGenome™ Universal Unmethylated DNA Set provides 2 separate genomic DNA controls for gene methylation studies. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) and can be subsequently used as an unmethylated DNA control for gene methylation studies.

The CpGenome™ DNA Modification Kit (Cat. No. S7820) contains the reagents necessary to perform the initial bisulfite reactions, while the CpG WIZ® BRCA1 Amplification Kit contains the reagents required for the PCR amplification reactions.

With a uniform thermal ramp rate of over 10°C per second xxpress™ is the fastest real-time thermocycler in the World delivering 40 cycle q-PCR in under 10 minutes. And whilst not all PCR chemistries are yet as fast as this system there are a number that repeatably and reliably work at this speed. Ultra low thermal mass and high conductively enable the patented resistive heating system deliver this speed cycle more efficiently…

An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…

A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…

Our uncloned normalized cDNA is created using proprietary technology which features full-length-enriched and high quality size-fractionated cDNA. Our cDNA has been used successfully with FLX, Titanium an Illumina sequencing.