PCR and Thermal Cycling Products & Reviews

2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference d

Brighter signal for earlier quantification of low and high copy RNA targets in a convenient 1-Step RT-qPCR format GoTaq® 1-Step RT-qPCR System is a reagent system that gives a brighter signal for earlier quantification of low and high copy RNA targets, using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube format. The system incorporates BRYT Green® Fluorescent Dye, a novel dye…

A Billion Reactions. Digital Answers. Experience the Next Generation of PCR. Capable of generating more than a billion reactions in a single day, the RainDrop™ Digital PCR System surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation, and multiplexing.Based on RainDance’s proven picodroplet platform, the RainDrop System delivers absolute quantitation of target molecules. Th…

Enclosed – safe – sound: Xiril’s Neon100 with Hood Xiril liquid handling robots are ideally suited to perform a large number of liquid handling tasks in automation solutions. E.g. processes such as nucleic acid separation or PCR setup can easily be automated. The proven pipetting robot Neon is available with up to 36 positions on the worktable, carrying one or two robotic arms facilitating your daily pipetting routine. The bas…

A 38µm soft non-permeable aluminum foil sealing film with strong medical grade adhesive, AlumaSeal II™ sealing films eliminate the need for heat-sealing devices or mats during thermal cycling. Compared to other aluminum foils, AlumaSeal II™ has less tendency to roll back on itself when removing the backing paper and conforms well to the plate during applications. •   Easily pierceable with single or multi-channel pipettors and…

The PowerPlex® Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples.   The kit contains: all 12 loci in the current PowerPlex® Y System, the additional 5 loci found in AmpFlSTR® Y-filer®, plus 6 new loci.   PowerPlex® Y23 System Benefits: Reveal more Y-STR loci in excess female DNA Cut your amplification time roughly in ha…

Made of thin wall polypropylene and designed to fit on all Corning® Thermowell™ GOLD 1 x 8 well PCR strips and 96 well PCR microplates DNase- and RNase-free Autoclavable at 121°C

Thermo Scientific* Fermentas PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs and all the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR. ■ Convenient—Taq DNA Polymerase in a ready-to-use mix ■ Fast—sa…

The MagNA Pure LC Cooling Block, LC Centrifuge Adapters, holds 32 LightCycler® Centrifuge Adapters containing LightCycler® Capillaries (20 µl). The Cooling Block is designed to cool the capillaries during automated PCR setup by the MagNA Pure LC Instrument. In addition, the Cooling Block holds fifteen 1.5 ml reaction tubes (e.g., for master mixes). The stage of the MagNA Pure LC Instrument includes a cooling area, which is des…

The MagNA Pure LC Cooling Block, LC Sample Carousel holds one LightCycler® Sample Carousel (all formats) containing LightCycler® Capillaries. The Cooling Block is designed to cool the capillaries during automated PCR setup by the MagNA Pure LC Instrument. In addition, the Cooling Block holds sixteen 1.5 ml reaction tubes (e.g. for master mixes). The stage of the MagNA Pure LC Instrument includes a cooling area, which is design…

Application The MagNA Pure LC Cooling Block, Reaction Tubes, is used for placing thirty-two 1.5 ml reaction tubes, with screw caps, on the stage of the MagNA Pure LC Instrument. This Cooling Block can be combined with either the MagNA Pure LC Cooling Block, LC Centrifuge Adapters, or 96-well PCR Plate. The stage of the MagNA Pure LC Instrument includes a cooling area that is designed for various post-elution pipetting proces…

Application The MagNA Pure LC Cooling Block, A-Ring holds one or two A-Rings with bar codes. Application of the corresponding Post Elution Module of the MagNA Pure LC Software results in automatic filling of the A-Rings with purified nucleic acids and compatible PCR reagents, which are also positioned on the Cooling Block. The A-Rings are then ready to be transferred to an instrument platform for testing. The stage of th…

Easy-to-use, one-component reagent for internal labeling of oligonucleotide probes to be detected on the LightCycler® and LightTyper Instruments. Application The SimpleProbe 519 Labeling Reagent is used for the internal labeling of oligonucleotide probes that are to be detected at  519 nm, for example, when used as mutation detection probes on the LightCycler® and LightTyper Systems following the SimpleProbe probe principle.…

GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. This system consists of a base module and one of many interchangeable sample block modules. The Auto-Lid Dual 384-well GeneAmp® PCR System 9700 is designed for use with 384-well reaction plates for high-throughput PCR and cycle sequencing applications. Automated heated lid delivers precision movement with a 15-second opening and closing t…

GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The Auto-Lid Dual 384-well GeneAmp® PCR System 9700 is designed for use with 384-well reaction plates for high-throughput PCR and cycle sequencing applications. • Compact design to maximize bench space • Two 384-well sample blocks…

The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format.   It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add…

The GoTaq® Probe 2-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system protocol facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: • GoScript™ Reverse Transcription System • GoTaq® Probe qPCR Master Mix The GoScript™ Reverse Transcription System includes an optimized reaction b…

The new Mastercycler nexus X1 combines the modern and intuitive software from the Mastercycler nexus with a fast 96-well silver block for increased heating and cooling rates. It is fast, easy to use, does not need much space or energy and sends you an e-mail when it is done—what else do you need from a PCR cycler? Combine, Connect, Control • Combine up to 3 units for maximum throughput! • Connect your Mastercycler nexus to you…

LightCycler® Nano Instrument - Real-time PCR amplification and detection instrument for tube-based assays of up to 32 (4 x 8) samples per run, with minimal noise, footprint and weight. Assembled from just a few building blocks, the robust LightCycler® Nano System is easy to transport and install. Up to 32 samples can be run in 0.2 ml tubes connected as strips. Fast heating and cooling is done using Peltier elements. Assay per…

Real-time fluorescent Campylobacter detection

The Thermo Scientific* SureTect Listeria monocytogenes PCR Assay is a multiplex real-time assay that incorporates unique primer and probe components for highly-specific detection of the prfA gene, specific to and found only in Listeria monocytogene.The assay also includes primer and probe components, for simultaneous detection of an internal amplification control (IAC), for confirmation of successful PCR and increased confiden…

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. The Phire® Animal Tissue Direct PCR Kit employs Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA-binding domain that enhances the processivity of the polymerase. Phire Hot Start II DNA Polymerase also exhibits extreme…

Thermo Scientific Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to PCR inhibitors present in blood and retains its activity even with samples containing 40% whole blood. This extremely accurate proofreading DNA polymerase makes the Phusion B…

If there is DNA to detect, the Plexor® HY System will show it. Total human DNA. Total male DNA. The power to see it all in a single assay.   The Plexor® HY System is a real-time PCR assay to determine the concentration of total human DNA and male human DNA simultaneously in one reaction. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors…

Pre-designed, gene-specific probe and primer pairs for repeatable, sensitive and gene specific RT-qPCR quantification for human and mouse protein-coding genes and long noncoding RNAs (lncRNAs). Thermo Scientific Solaris qPCR Gene Expression Assays are pre-designed, gene specific probe and primer pairs that utilize minor groove binder (MGB) and Superbase technologies to deliver repeatable, sensitive and gene specific RT-qPCR (…