Takara produce a host of products for PCR Takara’s new sensitive qPCR premixes contains TLi RNase H and are developed to address the inhibitory effect on the PCR due to the presence of remaining RNA after cDNA synthesis. PrimeSTAR®, a non-Taq polymerase with high proofreading activity are now available in a high speed version, PrimeSTAR Max, with extension rate of less than 5 sek/kb and PrimeSTAR GXL, that amplifies up to 30 k…
PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR. Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension,…
SapphireAmp Fast PCR Master Mix is a hot start PCR master mix that contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a convenient 2X premix. SapphireAmp PCR Master Mix is especially designed for fast PCR reactions that can be completed in half the time required when using conventional Taq DNA polymerase. For human genomic DNA, amplification of 2 kb targets can be…
PrimeSTAR GXL DNA Polymerase is a high fidelity polymerase that provides efficient PCR amplification even in challenging situations, such as when amplifiying GC-rich templates, when there is eXcess template in the reaction mixture, and when amplifying Long products up to 30 kb (GXL). PrimeSTAR GXL Polymerase is the most robust high fidelity polymerase available, routinely allowing amplification of PCR products that cannot be…
The Takara Bio TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies.
Real Time RT-PCR: One-Step PrimeScript RT-PCR Kit (Perfect Real Time)
A High Fidelity PCR Enzyme for Long Range PCR: LA Taq DNA Polymerase
The EmeraldAmp MAX PCR Master Mix ensures PCR specificity and includes an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green) and a density reagent in a convenient 2X premix format. Only primer and DNA template need to be added to initiate the reaction. This premix can be used for routine PCR applications as well as those requiring PCR specificity. Completed reactions are capable of being analyzed directly via…
The Magnosphere UltraPure mRNA Purification Kit is designed for mRNA purification from total RNA derived from cultured cells or animal tissues. This mRNA isolation kit includes Magnosphere oligo-dT beads (JSR Life Sciences Corporation) that are capable of isolating intact, high-purity polyA+ RNA with an extremely low rate of ribosomal RNA (rRNA) contamination. The beads have a uniform particle size and super paramagnetism, al…
Components : Tks Gflex DNA Polymerase 250 U 2 X Gflex PCR Buffer (Mg2+, dNTP plus) 1 ml x 5 Mg2+ conc. (2 X) 2 mM dNTP conc. (2 X) ea. 400 μM
The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enable…
This Takara Bio SYBR Green Master Mix is recommended for real-time PCR (qPCR) when high specificity is critical, because this reagent effectively suppresses amplification of primer-dimers and off-target products. Use this product when primer design has been optimized, but an increase in specificity of the qPCR assay is still needed. SYBR Premix Ex Taq II (Tli RNase H Plus) includes SYBR Green I, a reagent designed for interca…
DEXPAT Reagent from Takara Bio is designed for one step DNA extraction from paraffin embedded tissue fixed in 10% formalin. DEXPAT Reagent eliminates the laborious deparaffinization, organic solvent extraction and enzymatic digestion steps required in most standard DNA extraction protocols. Furthermore, DEXPAT Reagent enables preparation of PCR-ready DNA in only 25 minutes versus 2-3 days using conventional DNA extraction met…
The EpiScope MSP Kit features a reagent designed exclusively for DNA methylation analysis via methylation specific PCR (MSP). This reagent contains a specific enzyme in an optimized buffer, allowing MSP analyses on bisulfite-treated uracil-containing DNA templates. This kit features high amplification efficiency and methylation specificity compared with standard PCR. The EpiScope MSP Kit is optimized for real-time monitoring…
Generate cDNA for real-time PCR: PrimeScript RT Reagent Kit (Perfect Real Time)
cDNA synthesis kit: PrimeScript First Strand cDNA Synthesis Kit
GenomeWalker™ Kits provide a simple, PCR-based method for walking upstream or downstream in genomic DNA from a known sequence, such as an expressed sequence tag (EST). GenomeWalker allows you to take individual steps of up to 6 kb in genomic DNA using long-distance PCR. Walks can be extended simply by taking multiple steps using new primers based on the sequence obtained in previous steps.
