Takara produce a host of products for PCR Takara’s new sensitive qPCR premixes contains TLi RNase H and are developed to address the inhibitory effect on the PCR due to the presence of remaining RNA after cDNA synthesis. PrimeSTAR®, a non-Taq polymerase with high proofreading activity are now available in a high speed version, PrimeSTAR Max, with extension rate of less than 5 sek/kb and PrimeSTAR GXL, that amplifies up to 30 k…
PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR. Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension,…
SapphireAmp Fast PCR Master Mix is a hot start PCR master mix that contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a convenient 2X premix. SapphireAmp PCR Master Mix is especially designed for fast PCR reactions that can be completed in half the time required when using conventional Taq DNA polymerase. For human genomic DNA, amplification of 2 kb targets can be…
PrimeSTAR GXL DNA Polymerase is a high fidelity polymerase that provides efficient PCR amplification even in challenging situations, such as when amplifiying GC-rich templates, when there is eXcess template in the reaction mixture, and when amplifying Long products up to 30 kb (GXL). PrimeSTAR GXL Polymerase is the most robust high fidelity polymerase available, routinely allowing amplification of PCR products that cannot be…
The Takara Bio TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies.
Real Time RT-PCR: One-Step PrimeScript RT-PCR Kit (Perfect Real Time)
A High Fidelity PCR Enzyme for Long Range PCR: LA Taq DNA Polymerase
PCR Cloning Kit-Seamless DNA Cloning with In-Fusion HD Cloning Plus
These bacterial expression vectors carry the gene for either GFP (wild type Aequorea victoria green fluorescent protein) or GFPuv (a GFP variant optimized for maximal fluorescence when excited by UV light [360–400 nm]) and are driven by the lac promoter. These vectors can also be used as a source of the indicated fluorescent protein coding sequences. The fluorescent protein gene can be amplified by PCR or readily excised using…
The EmeraldAmp MAX PCR Master Mix ensures PCR specificity and includes an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green) and a density reagent in a convenient 2X premix format. Only primer and DNA template need to be added to initiate the reaction. This premix can be used for routine PCR applications as well as those requiring PCR specificity. Completed reactions are capable of being analyzed directly via…
SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing—Single-Cell Transcriptome Analysis with Ultimate Sensitivity
The DNA Ligation Kit Mighty Mix is a single solution, ready-to-use 2X ligation mix that enables high efficiency ligations, particularly for blunt-ended ligations. This DNA ligation kit can also be used for ligation into TA-vectors. The basic ligation reaction can be quickly performed, either for 5 minutes at 25°C or for 30 minutes at 16°C, with equal results for both circular and linear DNA ligations. The 2X Ligation Mix is c…
The Magnosphere UltraPure mRNA Purification Kit is designed for mRNA purification from total RNA derived from cultured cells or animal tissues. This mRNA isolation kit includes Magnosphere oligo-dT beads (JSR Life Sciences Corporation) that are capable of isolating intact, high-purity polyA+ RNA with an extremely low rate of ribosomal RNA (rRNA) contamination. The beads have a uniform particle size and super paramagnetism, al…
Chemically defined human iPS cell culture medium for preclinical use.
Components : Tks Gflex DNA Polymerase 250 U 2 X Gflex PCR Buffer (Mg2+, dNTP plus) 1 ml x 5 Mg2+ conc. (2 X) 2 mM dNTP conc. (2 X) ea. 400 μM
More than 18,000 studies have been published citing the use of the yeast two-hybrid system to investigate protein-protein interactions, and Clontech’s Matchmaker™ Systems have been by far the most popular. Matchmaker™ Gold Systems are the highest performing incarnations of our Matchmaker product line, due to a combination of a new yeast strain (Y2HGold), stringent reporters, easy to use libraries, high-level expression vectors…
The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enable…
This Takara Bio SYBR Green Master Mix is recommended for real-time PCR (qPCR) when high specificity is critical, because this reagent effectively suppresses amplification of primer-dimers and off-target products. Use this product when primer design has been optimized, but an increase in specificity of the qPCR assay is still needed. SYBR Premix Ex Taq II (Tli RNase H Plus) includes SYBR Green I, a reagent designed for interca…
Research and Clinical Grade RetroNectin Reagent for Retroviral or Lentiviral Transduction. RetroNectin Reagent for retroviral or lentiviral transduction significantly increases transduction efficiency and results in enhanced gene transfer, especially in suspension and hematopoietic stem cells. RetroNectin Reagent is a chimeric peptide composed of E. coli-derived recombinant human fibronectin fragment that contains three funct…
Flow Cytometer Calibration Beads for AcGFP/EGFP and mCherry. Use these beads to calibrate your flow cytometer prior to analyzing cells that express the AcGFP1 or mCherry fluorescent proteins. The AcGFP Flow Cytometer Calibration Beads allow for easy calibration of any flow cytometer with a 488 nm laser line that excites the green fluorescent proteins AcGFP1 (Aequorea coerulescens GFP) and EGFP. [The AcGFP Flow Cytometer Calib…
